{"id":1031,"date":"2015-07-02T18:06:03","date_gmt":"2015-07-02T09:06:03","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=1031"},"modified":"2015-07-02T18:10:34","modified_gmt":"2015-07-02T09:10:34","slug":"journal-club-2015-7-3","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=1031","title":{"rendered":"Journal club 2015.7.3"},"content":{"rendered":"

Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing<\/span><\/a><\/h1>\n
\n
Usoskin D<\/span><\/a>1<\/span><\/sup>, Furlan A<\/span><\/a>1<\/span><\/sup>, Islam S<\/span><\/a>1<\/span><\/sup>, Abdo H<\/span><\/a>1<\/span><\/sup>, L\u00f6nnerberg P<\/span><\/a>1<\/span><\/sup>, Lou D<\/span><\/a>1<\/span><\/sup>, Hjerling-Leffler J<\/span><\/a>1<\/span><\/sup>, Haeggstr\u00f6m J<\/span><\/a>2<\/span><\/sup>, Kharchenko O<\/span><\/a>1<\/span><\/sup>, Kharchenko PV<\/span><\/a>3<\/span><\/sup>, Linnarsson S<\/span><\/a>1<\/span><\/sup>, Ernfors P<\/span><\/a>1<\/span><\/sup>.<\/div>\n
\n

Author information<\/span><\/span><\/a><\/h3>\n<\/div>\n
\n

Abstract<\/h3>\n
\n

The primary sensory<\/span> system requires the integrated function of multiple cell types<\/span>, although its full complexity remains unclear. We used comprehensive transcriptome analysis of 622 single mouse neurons to classify them in an unbiased<\/span> manner, independent of any a priori knowledge of sensory<\/span> subtypes. Our results reveal eleven types<\/span>: three distinct low-threshold mechanoreceptive neurons, two proprioceptive, and six principal types<\/span> of thermosensitive, itch sensitive, type C low-threshold mechanosensitive and nociceptive neurons with markedly different molecular and operational properties. Confirming previously anticipated major neuronal types<\/span>, our results also classify and provide markers for new, functionally distinct subtypes. For example, our results suggest that itching during inflammatory skin diseases such as atopic dermatitis is linked to a distinct itch-generating type. We demonstrate single-cell<\/span> RNA<\/span>-seq as an effective strategy for dissecting sensory<\/span> responsive cells into distinct neuronal types<\/span>. The resulting catalog illustrates the diversity of sensory<\/span> types<\/span> and the cellular complexity underlying somatic sensation.<\/p>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing Usoskin D1, Furlan A1, Islam S1, Abdo H1, L\u00f6nnerberg P1, Lou D1, Hjerling-Leffler J1, Haeggstr\u00f6m J2, Kharchenko O1, Kharchenko PV3, Linnarsson S1, Ernfors P1. Author information Abstract The primary sensory system requires the integrated function of multiple cell types, although its full complexity remains […]<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1031"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1031"}],"version-history":[{"count":4,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1031\/revisions"}],"predecessor-version":[{"id":1035,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1031\/revisions\/1035"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1031"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1031"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1031"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}