{"id":1167,"date":"2016-02-12T11:32:49","date_gmt":"2016-02-12T02:32:49","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=1167"},"modified":"2016-02-12T11:32:49","modified_gmt":"2016-02-12T02:32:49","slug":"journal-club-2016-02-12","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=1167","title":{"rendered":"Journal club 2016-02-12"},"content":{"rendered":"

TRPV1 and PLC Participate in Histamine H4 Receptor-Induced Itch<\/h2>\n
Tunyu Jian<\/a>,1<\/sup>\u00a0Niuniu Yang<\/a>,1<\/sup>\u00a0Yan Yang<\/a>,1<\/sup>\u00a0Chan Zhu<\/a>,1<\/sup>\u00a0Xiaolin Yuan<\/a>,1<\/sup>\u00a0Guang Yu<\/a>,1<\/sup>\u00a0Changming Wang<\/a>,1<\/sup>\u00a0Zhongli Wang<\/a>,1<\/sup>\u00a0Hao Shi<\/a>,1<\/sup>\u00a0Min Tang<\/a>,1<\/sup>\u00a0Qian He<\/a>,1<\/sup>\u00a0Lei Lan<\/a>,2<\/sup>\u00a0Guanyi Wu<\/a>,1,3<\/sup>\u00a0and Zongxiang Tang<\/a>1<\/sup><\/div>\n

1<\/sup>College of Basic Medicine, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, China
\n2<\/sup>College of Life Science, Nanjing Normal University, Nanjing 210046, China
\n3<\/sup>College of Basic Medicine, Guangxi University of Chinese Medicine, 13 Wuhe Road, Nanning 530200, China<\/p>\n

 <\/p>\n

TRPV1 and PLC Participate in Histamine H4 Receptor-Induced Itch<\/a><\/p>\n

<\/div>\n
\n

Abstract<\/h4>\n

Histamine H4 receptor has been confirmed to play a role in evoking peripheral pruritus. However, the ionic and intracellular signaling mechanism of activation of H4 receptor on the dorsal root ganglion (DRG) neurons is still unknown. By using cell culture and calcium imaging, we studied the underlying mechanism of activation of H4 receptor on the DRG neuron. Immepip dihydrobromide (immepip)\u2014a histamine H4 receptor special agonist under cutaneous injection\u2014obviously induced itch behavior of mice. Immepip-induced scratching behavior could be blocked by TRPV1 antagonist AMG9810 and PLC pathway inhibitor U73122. Application of immepip (8.3\u201350\u2009\u03bc<\/i>M) could also induce a dose-dependent increase in intracellular Ca2+<\/sup> () of DRG neurons. We found that 77.8% of the immepip-sensitized DRG neurons respond to the TRPV1 selective agonist capsaicin. U73122 could inhibit immepip-induced Ca2+<\/sup> responses. In addition, immepip-induced increase could be blocked by ruthenium red, capsazepine, and AMG9810; however it could not be blocked by TRPA1 antagonist HC-030031. These results indicate that TRPV1 but not TRPA1 is the important ion channel to induce the DRG neurons\u2019 responses in the downstream signaling pathway of histamine H4 receptor and suggest that TRPV1 may be involved in the mechanism of histamine-induced itch response by H4 receptor activation.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

TRPV1 and PLC Participate in Histamine H4 Receptor-Induced Itch Tunyu Jian,1\u00a0Niuniu Yang,1\u00a0Yan Yang,1\u00a0Chan Zhu,1\u00a0Xiaolin Yuan,1\u00a0Guang Yu,1\u00a0Changming Wang,1\u00a0Zhongli Wang,1\u00a0Hao Shi,1\u00a0Min Tang,1\u00a0Qian He,1\u00a0Lei Lan,2\u00a0Guanyi Wu,1,3\u00a0and Zongxiang Tang1 1College of Basic Medicine, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, China 2College of Life Science, Nanjing Normal University, Nanjing 210046, China 3College of Basic Medicine, Guangxi […]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1167"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1167"}],"version-history":[{"count":3,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1167\/revisions"}],"predecessor-version":[{"id":1172,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1167\/revisions\/1172"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1167"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1167"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1167"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}