{"id":1173,"date":"2016-02-19T10:37:11","date_gmt":"2016-02-19T01:37:11","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=1173"},"modified":"2016-02-19T10:37:11","modified_gmt":"2016-02-19T01:37:11","slug":"journal-club-2016-02-19","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=1173","title":{"rendered":"Journal club 2016-02-19"},"content":{"rendered":"

Voltage-Gated Potassium Channels Involved in Regulation of Physiological Function in MrgprA3-Specific Itch Neurons.<\/h1>\n
Tang M<\/span><\/a>1<\/span><\/sup>, Wu G<\/span><\/a>2<\/span><\/sup>, Wang Z<\/span><\/a>3<\/span><\/sup>, Yang N<\/span><\/a>3<\/span><\/sup>, Shi H<\/span><\/a>3<\/span><\/sup>, He Q<\/span><\/a>4<\/span><\/sup>, Zhu C<\/span><\/a>3<\/span><\/sup>, Yang Y<\/span><\/a>3<\/span><\/sup>, Yu G<\/span><\/a>3<\/span><\/sup>, Wang C<\/span><\/a>3<\/span><\/sup>, Yuan X<\/span><\/a>3<\/span><\/sup>, Liu Q<\/span><\/a>5<\/span><\/sup>, Guan Y<\/span><\/a>6<\/span><\/sup>, Dong X<\/span><\/a>7<\/span><\/sup>, Tang Z<\/span><\/a>8<\/span><\/sup>.<\/div>\n
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Abstract<\/h3>\n
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Itch is described as an unpleasant or irritating skin sensation that elicits the desire or reflex to scratch. MrgprA3, one of members of the Mrgprs family, is specifically expressed in a subpopulation of dorsal root ganglion (DRG) in the peripheral nervous system (PNS). These MrgprA3-expressing DRG neurons have been identified as itch-specific neurons. They can be activated by the compound, chloroquine, which is used as a drug to treat malaria. In the present study, we labeled these itch-specific neurons using the method of molecular genetic markers, and then studied their electrophysiological properties. We also recorded the cutaneous MrgprA3–<\/span><\/sup> neurons retrogradely labeled by Dil dye (MrgprA3–<\/span><\/sup>-Dil). We first found that MrgprA3+<\/span><\/sup> neurons have a lower excitability than MrgprA3–<\/span><\/sup> neurons (MrgprA3–<\/span><\/sup>-non-Dil and MrgprA3–<\/span><\/sup>-Dil). The number of action potential (AP) was reduced more obviously in MrgprA3+<\/span><\/sup> neurons than that of in MrgprA3–<\/span><\/sup> neurons. In most cases, MrgprA3+<\/span><\/sup> neurons only generated single AP; however, in MrgprA3–<\/span><\/sup> neurons, the same stimulation could induce multiple AP firing due to the greater voltage-gated potassium (Kv) current existence in MrgprA3+<\/span><\/sup> than in MrgprA3–<\/span><\/sup> neurons. Thus, Kv current plays an important role in the regulation of excitability in itch-specific neurons.<\/p>\n

Copyright \u00a9 2016. Published by Elsevier B.V.<\/p>\n<\/div>\n<\/div>\n

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KEYWORDS:<\/h4>\n

DRG; Itch; Kv current; MrgprA3<\/p>\n

 <\/p>\n

voltage gated K+ channel and Mrgpra3 specific itchneuron<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Voltage-Gated Potassium Channels Involved in Regulation of Physiological Function in MrgprA3-Specific Itch Neurons. Tang M1, Wu G2, Wang Z3, Yang N3, Shi H3, He Q4, Zhu C3, Yang Y3, Yu G3, Wang C3, Yuan X3, Liu Q5, Guan Y6, Dong X7, Tang Z8. Abstract Itch is described as an unpleasant or irritating skin sensation that […]<\/p>\n","protected":false},"author":6,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1173"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1173"}],"version-history":[{"count":2,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1173\/revisions"}],"predecessor-version":[{"id":1176,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1173\/revisions\/1176"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1173"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1173"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1173"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}