{"id":1387,"date":"2017-03-10T08:16:32","date_gmt":"2017-03-09T23:16:32","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=1387"},"modified":"2017-03-19T22:55:46","modified_gmt":"2017-03-19T13:55:46","slug":"journal-club-2017-03-10","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=1387","title":{"rendered":"Journal Club 2017. 03. 10."},"content":{"rendered":"<h1><strong>Mechanisms of Pruritogen-Induced Activation of Itch Nerves in Isolated Mouse Skin<\/strong><\/h1>\n<p>Ru, F1., Sun, H1., Jurcakova, D.1,3, Herbstsomer, R.A1., Meixong, J2., Dong, X2, Undem, B.J. 1<br \/>\nDepartments of Medicine1 and Neuroscience2, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA, 3Department of Pathophysiology, Biomedical Center Martin, Jessenius Medical School, Comenius University, Martin, Slovakia<\/p>\n<p>Running Title: Characterization of itch causing nerves<\/p>\n<p><a href=\"http:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2017\/03\/Mechanisms-of-Pruritogen-Induced-Activation-of-Itch-Nerves-in-Isolated-Mouse-Skin.pdf\">Mechanisms of Pruritogen-Induced Activation of Itch Nerves in Isolated Mouse Skin<\/a><\/p>\n<p>Key Points Summary<br \/>\nChloroquine (CQ) stimulates itch nerves and causes intense scratching in mice by activating the G- Protein Coupled Receptor (GPCR) MrgprA3. It is not known how stimulation of MrgprA3 (or other GPCRs) leads to activation of the itch nerve terminals in the skin, but previous studies have found that TRPA1 gene deletion blocks CQ-induced scratching.<br \/>\nIn the present study we used a novel dorsal skin-nerve preparation to evaluate mechanisms underlying CQ- and histamine-induced action potential discharge in itch nerve terminals.<br \/>\nWe found that CQ activation of the nerves requires the beta3 isoform of phospholipase C, however, TRPA1 or other TRP channel are not required. Evidence is provided for a role for calcium-activated chloride channels such as TMEM16a in GPCR-activation of itch nerve terminals.<br \/>\nThe mechanism by which TRP channels participate in pruritogen-induced scratching may involve sites of action other than the primary afferent terminals.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Mechanisms of Pruritogen-Induced Activation of Itch Nerves in Isolated Mouse Skin Ru, F1., Sun, H1., Jurcakova, D.1,3, Herbstsomer, R.A1., Meixong, J2., Dong, X2, Undem, B.J. 1 Departments of Medicine1 and Neuroscience2, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA, 3Department of Pathophysiology, Biomedical Center Martin, Jessenius Medical School, Comenius University, Martin, Slovakia Running Title: [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1387"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1387"}],"version-history":[{"count":3,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1387\/revisions"}],"predecessor-version":[{"id":1393,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1387\/revisions\/1393"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1387"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1387"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1387"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}