{"id":1544,"date":"2017-11-24T10:45:44","date_gmt":"2017-11-24T01:45:44","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=1544"},"modified":"2017-11-24T10:45:44","modified_gmt":"2017-11-24T01:45:44","slug":"journal-club-17-11-24","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=1544","title":{"rendered":"Journal Club 17.11.24"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>Involvement of thromboxane A2 in interleukin-31-induced itch-associated response in mice<\/p>\n<table class=\"profiletable\" style=\"height: 1110px\" border=\"1\" summary=\"\uc774 \ud45c\ub294 \uc11c\uc9c0\uc0c1\uc138\uc815\ubcf4\ub97c \ub098\ud0c0\ub0b4\uace0 \uc788\uc2b5\ub2c8\ub2e4.\" width=\"629\" cellspacing=\"0\">\n<tbody>\n<tr>\n<th>\uc800\uc790<\/th>\n<td><a href=\"http:\/\/lib.gachon.ac.kr\/eds\/brief\/discoveryResult?st=KWRD&amp;service_type=brief&amp;si=AU&amp;q=%22Andoh%2C+Tsugunobu%22\"><span style=\"color: #0066cc\">Andoh, Tsugunobu <sup><span style=\"font-size: small\">\u204e<\/span><\/sup><\/span><\/a><br \/>\n<a href=\"http:\/\/lib.gachon.ac.kr\/eds\/brief\/discoveryResult?st=KWRD&amp;service_type=brief&amp;si=AU&amp;q=%22Li%2C+Sikai%22\"><span style=\"color: #0066cc\">Li, Sikai<\/span><\/a><br \/>\n<a href=\"http:\/\/lib.gachon.ac.kr\/eds\/brief\/discoveryResult?st=KWRD&amp;service_type=brief&amp;si=AU&amp;q=%22Uta%2C+Daisuke%22\"><span style=\"color: #0066cc\">Uta, Daisuke<\/span><\/a><\/td>\n<\/tr>\n<tr>\n<th>\uc790\ub8cc\uc720\ud615<\/th>\n<td>Article<\/td>\n<\/tr>\n<tr>\n<th>\ucd08\ub85d<\/th>\n<td>\u2022Interleukinin-31 (IL-31) induced scratching, an itch-associated response, in mice.\u2022IL-31-induced scratching was inhibited by TP thromboxane (TX) receptor antagonist.\u2022IL-31 receptors were expressed mainly in epidermal keratinocytes.\u2022IL-31 induced TXA2 production in the skin and primary cultures of keratinocytes.\u2022The results suggest that IL-31 elicits scratching via epidermal TXA2 production.<br \/>\nBackground Atopic dermatitis is a chronic and severe pruritic skin disease. Interlukin-31 (IL-31) has been recently demonstrated to be one of the key pruritogens in atopic dermatitis. However, the mechanisms underlying IL-31-induced itching remains unclear. In our previous study, we have shown that thromboxane (TX) A2 is involved in itch-associated responses in mice with atopy-like skin diseases.<br \/>\nMethods IL-31 was given intradermally into the rostral back of ICR mice and the hind-paw scratching to the injection site were counted. Expression of TX synthase and IL-31 receptors were analyzed using immunohistochemical staining or RT-PCR in mouse skin or primary cultures of mouse keratinocytes. The concentration of TXB2, a metabolite of TXA2, in the skin and the culture medium of primary cultures of mouse keratinocytes was measured using enzyme immunoassay. The concentration of intracellular Ca<sup><span style=\"font-size: small\">2+<\/span><\/sup> ions in mouse keratinocytes was measured using the calcium imaging method.<br \/>\nResults An intradermal injection of IL-31 elicited scratching, an itch-related response, in mice. The scratching was inhibited by TP TXA2 receptor antagonist DCHCH. The distribution of TX synthase and IL-31RA receptor was mainly epidermal keratinocytes in the skin. The primary cultures of keratinocytes expressed the mRNAs of TX synthase and IL-31 receptors. IL-31 increased the concentration of TXB2, which was inhibited by TX synthase inhibitor sodium ozagrel and EGTA, in the skin and the culture medium of primary cultures of keratinocytes. IL-31 increased the concentration of intracellular Ca<sup><span style=\"font-size: small\">2+<\/span><\/sup> ions in mouse keratinocytes.<br \/>\nConclusion It is suggested that IL-31 elicits itch-associated responses through TXA2 produced from keratinocytes.<\/td>\n<\/tr>\n<tr>\n<th>Affiliation<\/th>\n<td>Department of Applied Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan<\/td>\n<\/tr>\n<tr>\n<th>Publisher<\/th>\n<td>Elsevier Urban &amp; Partner Sp. z o.o.<\/td>\n<\/tr>\n<tr>\n<th>DOI<\/th>\n<td>10.1016\/j.pharep.2017.10.001<\/td>\n<\/tr>\n<tr>\n<th>Accession Number<\/th>\n<td>S1734114017303808<\/td>\n<\/tr>\n<tr>\n<th>Copyright<\/th>\n<td>\u00a9 2017 Published by Elsevier Sp. z o.o. on behalf of Institute of Pharmacology, Polish Academy of Sciences.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><a href=\"http:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2017\/11\/Involvement-of-thromboxane-A2-in-interleukin-31-induced-itch-associated-response-in-mice.pdf\">Involvement of thromboxane A2 in interleukin-31-induced itch-associated response in mice<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<table class=\"profiletable\" style=\"height: 1110px\" border=\"1\" summary=\"\uc774 \ud45c\ub294 \uc11c\uc9c0\uc0c1\uc138\uc815\ubcf4\ub97c \ub098\ud0c0\ub0b4\uace0 \uc788\uc2b5\ub2c8\ub2e4.\" width=\"629\" cellspacing=\"0\">\n<tbody>\n<tr>\n<th><\/th>\n<\/tr>\n<tr>\n<td>&nbsp;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; &nbsp; Involvement of thromboxane A2 in interleukin-31-induced itch-associated response in mice \uc800\uc790 Andoh, Tsugunobu \u204e Li, Sikai Uta, Daisuke \uc790\ub8cc\uc720\ud615 Article \ucd08\ub85d \u2022Interleukinin-31 (IL-31) induced scratching, an itch-associated response, in mice.\u2022IL-31-induced scratching was inhibited by TP thromboxane (TX) receptor antagonist.\u2022IL-31 receptors were expressed mainly in epidermal keratinocytes.\u2022IL-31 induced TXA2 production in the skin and [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1544"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1544"}],"version-history":[{"count":1,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1544\/revisions"}],"predecessor-version":[{"id":1546,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1544\/revisions\/1546"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1544"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1544"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1544"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}