{"id":1794,"date":"2019-09-06T10:40:25","date_gmt":"2019-09-06T01:40:25","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=1794"},"modified":"2019-09-06T10:40:28","modified_gmt":"2019-09-06T01:40:28","slug":"journal-club-19-09-06","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=1794","title":{"rendered":"Journal Club 19.09.06."},"content":{"rendered":"\n<h1 id=\"screen-reader-main-title\">Role of SNAREs in Atopic Dermatitis\u2013Related Cytokine Secretion and Skin-Nerve Communication<\/h1>\n\n\n\n<div class=\"wp-block-file\"><a href=\"http:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2019\/09\/Role-of-SNAREs-in-Atopic-Dermatitis-Rel...pdf\">Role-of-SNAREs-in-Atopic-Dermatitis-Rel..<\/a><a href=\"http:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2019\/09\/Role-of-SNAREs-in-Atopic-Dermatitis-Rel...pdf\" class=\"wp-block-file__button\" download>Download<\/a><\/div>\n\n\n\n<p><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0022202X19315593?via%3Dihub#!\">JianghuiMeng<sup>1<\/sup><sup>2<\/sup><\/a><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0022202X19315593?via%3Dihub#!\">JiafuWang<sup>1<\/sup><\/a><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0022202X19315593?via%3Dihub#!\">JoergBuddenkotte<sup>3<\/sup><sup>4<\/sup><\/a><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0022202X19315593?via%3Dihub#!\">TimoBuhl<sup>5<\/sup><\/a><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0022202X19315593?via%3Dihub#!\">MartinSteinhoff<sup>2<\/sup><sup>3<\/sup><sup>4<\/sup><sup>6<\/sup><\/a><\/p>\n\n\n\n<p>The role of soluble&nbsp;<em>N<\/em>-ethylmaleimide-sensitive factor attachment protein receptors in atopic dermatitis (AD) is unknown. This study identifies the function of soluble&nbsp;<em>N-<\/em>ethylmaleimide sensitive factor attachment protein receptor in AD-related cytokine secretion and epidermis-nerve communication. Herein, we report that various cytokines were simultaneously upregulated and coreleased in innate immunity\u2013activated primary human keratinocytes. AD-related cytokines thymic stromal lymphopoietin, endothelin-1, and inflammatory tumor necrosis factor-\u03b1 activated distinct but overlapping sensory neurons. Tumor necrosis factor-\u03b1 potentiated thymic stromal lymphopoietin\u2013induced Ca<sup>2+<\/sup>-influx, whereas endothelin-1 caused itch-selective B-type natriuretic peptide release. In primary human keratinocytes, B-type natriuretic peptide upregulated genes promoting dermatological and neuroinflammatory diseases and conditions. VAMP3, SNAP-29, and syntaxin 4 proved important in driving cytokine release from primary human keratinocytes. Depletion of VAMP3 inhibited nearly all the cytokine release including thymic stromal lymphopoietin and endothelin-1. Accordingly, VAMP3 co-occurred with endothelin-1 in the skins of patients with AD. Our study pinpoints the pivotal role of soluble&nbsp;<em>N-<\/em>ethylmaleimide sensitive factor attachment protein receptors in mediating cytokine secretion related to AD. VAMP3 is identified as a suitable target for developing broad-spectrum anticytokine therapeutics for controlling itch and atopic skin inflammation.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Role of SNAREs in Atopic Dermatitis\u2013Related Cytokine Secretion and Skin-Nerve Communication JianghuiMeng12JiafuWang1JoergBuddenkotte34TimoBuhl5MartinSteinhoff2346 The role of soluble&nbsp;N-ethylmaleimide-sensitive factor attachment protein receptors in atopic dermatitis (AD) is unknown. This study identifies the function of soluble&nbsp;N-ethylmaleimide sensitive factor attachment protein receptor in AD-related cytokine secretion and epidermis-nerve communication. Herein, we report that various cytokines were simultaneously upregulated and [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1794"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1794"}],"version-history":[{"count":1,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1794\/revisions"}],"predecessor-version":[{"id":1796,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1794\/revisions\/1796"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1794"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1794"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1794"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}