Tseng, Pang-Yen PhDa<\/sup>; Zheng, Qin PhDa<\/sup>; Li, Zhea<\/sup>; Dong, Xinzhong PhDa,b,<\/sup><\/p>\n\n\n\n Abstract<\/strong><\/p>\n\n\n\n In this study, we sought to elucidate the molecular mechanism underlying human Mas-related G protein\u2013coupled receptor X1 (MrgprX1)-mediated\u00a0itch\u00a0sensation. We found that activation of\u00a0MrgprX1\u00a0by\u00a0BAM8-22\u00a0triggered robust action potential discharges in\u00a0dorsal root ganglion neurons. This neuronal excitability is not mediated by transient receptor potential (TRP) cation channels, M-type potassium channels, or chloride channels. Instead, activation of\u00a0MrgprX1\u00a0lowers the activation threshold of tetrodotoxin-resistant sodium channels and induces inward sodium currents. These\u00a0MrgprX1-elicited action potential discharges can be blocked by Pertussis toxin and a G\u03b2\u03b3 inhibitor\u2014Gallein. Behavioral results showed that Nav1.9 knockout but not\u00a0TRPA1\u00a0knockout significantly reduced\u00a0BAM8-22\u00a0evoked scratching behavior. Collectively, these data suggest that activation of\u00a0MrgprX1\u00a0triggers\u00a0itch\u00a0sensation by increasing the activity of tetrodotoxin-resistant voltage-gated sodium channels.<\/p>\n\n\n\n Keywords:\u00a0MrgprX1, BAM8-22, Itch, Dorsal root ganglion neurons, TTX-resistant sodium channels, TRPA1, TRPV1<\/p>\n\n\n\n MrgprX1 mediates neuronal excitability and itch through tetrodotoxin-resistant sodium channels Tseng, Pang-Yen PhDa; Zheng, Qin PhDa; Li, Zhea; Dong, Xinzhong PhDa,b, Abstract In this study, we sought to elucidate the molecular mechanism underlying human Mas-related G protein\u2013coupled receptor X1 (MrgprX1)-mediated\u00a0itch\u00a0sensation. We found that activation of\u00a0MrgprX1\u00a0by\u00a0BAM8-22\u00a0triggered robust action potential discharges in\u00a0dorsal root ganglion neurons. This neuronal excitability is not […]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1798"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1798"}],"version-history":[{"count":1,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1798\/revisions"}],"predecessor-version":[{"id":1800,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1798\/revisions\/1800"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1798"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1798"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1798"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}