Lou Beaulieu-Laroche<\/a>\u00a01<\/a><\/sup>,\u00a0Marine Christin<\/a>\u00a01<\/a><\/sup>,\u00a0Annmarie Donoghue<\/a>\u00a02<\/a><\/sup>,\u00a0Francina Agosti<\/a>\u00a03<\/a><\/sup>,\u00a0Noosha Yousefpour<\/a>\u00a04<\/a><\/sup>,\u00a0Hugues Petitjean<\/a>\u00a01<\/a><\/sup>,\u00a0Albena Davidova<\/a>\u00a01<\/a><\/sup>,\u00a0Craig Stanton<\/a>\u00a01<\/a><\/sup>,\u00a0Uzair Khan<\/a>\u00a05<\/a><\/sup>,\u00a0Connor Dietz<\/a>\u00a05<\/a><\/sup>,\u00a0Elise Faure<\/a>\u00a06<\/a><\/sup>,\u00a0Tarheen Fatima<\/a>\u00a01<\/a><\/sup>,\u00a0Amanda MacPherson<\/a>\u00a05<\/a><\/sup>,\u00a0Stephanie Mouchbahani-Constance<\/a>\u00a01<\/a><\/sup>,\u00a0Daniel G Bisson<\/a>\u00a07<\/a><\/sup>,\u00a0Lisbet Haglund<\/a>\u00a07<\/a><\/sup>,\u00a0Jean A Ouellet<\/a>\u00a08<\/a><\/sup>,\u00a0Laura S Stone<\/a>\u00a09<\/a><\/sup>,\u00a0Jonathan Samson<\/a>\u00a010<\/a><\/sup>,\u00a0Mary-Jo Smith<\/a>\u00a011<\/a><\/sup>,\u00a0Kjetil Ask<\/a>\u00a012<\/a><\/sup>,\u00a0Alfredo Ribeiro-da-Silva<\/a>\u00a04<\/a><\/sup>,\u00a0Rikard Blunck<\/a>\u00a013<\/a><\/sup>,\u00a0Kate Poole<\/a>\u00a014<\/a><\/sup>,\u00a0Emmanuel Bourinet<\/a>\u00a03<\/a><\/sup>,\u00a0Reza Sharif-Naeini<\/a>\u00a015<\/a><\/sup><\/p>\n\n\n\n Mechanotransduction, the conversion of mechanical stimuli into electrical signals, is a fundamental process underlying essential physiological functions such as touch and pain sensing, hearing, and proprioception. Although the mechanisms for some of these functions have been identified, the molecules essential to the sense of pain have remained elusive. Here we report identification of TACAN (Tmem120A), an ion channel involved in sensing mechanical pain. TACAN is expressed in a subset of nociceptors, and its heterologous expression increases mechanically evoked currents in cell lines. Purification and reconstitution of TACAN in synthetic lipids generates a functional ion channel. Finally, a nociceptor-specific inducible knockout of TACAN decreases the mechanosensitivity of nociceptors and reduces behavioral responses to painful mechanical stimuli but not to thermal or touch stimuli. We propose that TACAN is an ion channel that contributes to sensing mechanical pain.<\/p>\n\n\n\n Keywords:\u00a0<\/strong>TACAN; bilayer; ion channel; mechanosensitive; mechanotransduction; nociceptor; pain; patch clamp; pillar.<\/p>\n\n\n\n TACAN Is an Ion Channel Involved in Sensing Mechanical Pain Lou Beaulieu-Laroche\u00a01,\u00a0Marine Christin\u00a01,\u00a0Annmarie Donoghue\u00a02,\u00a0Francina Agosti\u00a03,\u00a0Noosha Yousefpour\u00a04,\u00a0Hugues Petitjean\u00a01,\u00a0Albena Davidova\u00a01,\u00a0Craig Stanton\u00a01,\u00a0Uzair Khan\u00a05,\u00a0Connor Dietz\u00a05,\u00a0Elise Faure\u00a06,\u00a0Tarheen Fatima\u00a01,\u00a0Amanda MacPherson\u00a05,\u00a0Stephanie Mouchbahani-Constance\u00a01,\u00a0Daniel G Bisson\u00a07,\u00a0Lisbet Haglund\u00a07,\u00a0Jean A Ouellet\u00a08,\u00a0Laura S Stone\u00a09,\u00a0Jonathan Samson\u00a010,\u00a0Mary-Jo Smith\u00a011,\u00a0Kjetil Ask\u00a012,\u00a0Alfredo Ribeiro-da-Silva\u00a04,\u00a0Rikard Blunck\u00a013,\u00a0Kate Poole\u00a014,\u00a0Emmanuel Bourinet\u00a03,\u00a0Reza Sharif-Naeini\u00a015 Abstract Mechanotransduction, the conversion of mechanical stimuli into electrical signals, is a fundamental process underlying […]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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