{"id":2246,"date":"2021-04-30T10:18:28","date_gmt":"2021-04-30T01:18:28","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=2246"},"modified":"2021-04-30T10:54:33","modified_gmt":"2021-04-30T01:54:33","slug":"journal-club-2021-04-30","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=2246","title":{"rendered":"Journal Club-2021.04.30"},"content":{"rendered":"\n<p>J Invest Dermatol.\u00a02021 Apr 1;S0022-202X(21)01129-5.\u00a0doi: 10.1016\/j.jid.2021.03.015.Online ahead of print.<\/p>\n\n\n\n<h1>Mechanisms of broad-band ultraviolet B irradiation-induced itch in mice<\/h1>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Cao+L&amp;cauthor_id=33812858\">Liang Cao<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-1\">1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Yue+X&amp;cauthor_id=33812858\">Xueping Yue<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-1\">1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zhao+Y&amp;cauthor_id=33812858\">Yonghui Zhao<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-1\">1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Du+L&amp;cauthor_id=33812858\">Lixia Du<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-1\">1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Xie+Z&amp;cauthor_id=33812858\">Zili Xie<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-1\">1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Yuan+Y&amp;cauthor_id=33812858\">Yi Yuan<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-1\">1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zhang+S&amp;cauthor_id=33812858\">Sha Zhang<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-2\">2<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Li+F&amp;cauthor_id=33812858\">Feng Li<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-2\">2<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Feng+J&amp;cauthor_id=33812858\">Jing Feng<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-3\">3<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Hu+H&amp;cauthor_id=33812858\">Hongzhen Hu<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33812858\/#affiliation-1\">1<\/a><\/sup><\/p>\n\n\n\n<ul id=\"full-view-identifiers\"><li>PMID:&nbsp;<strong>33812858<\/strong><\/li><li>DOI:&nbsp;<a target=\"_blank\" rel=\"noreferrer noopener\" href=\"https:\/\/doi.org\/10.1016\/j.jid.2021.03.015\">10.1016\/j.jid.2021.03.015<\/a><\/li><\/ul>\n\n\n\n<h2>Abstract<\/h2>\n\n\n\n<p>Although sunburn can produce severe uncontrollable itching, the underlying mechanisms of ultraviolet (UV) irradiation-induced itch are poorly understood because of a lack of experimental animal models of sunburn itch. Here we established a sunburn-related mouse model and found that Broad-band UVB (BB-UVB) irradiation elicited scratching but not wiping behavior in mice. By using a combination of live-cell Ca<sup>2+<\/sup>&nbsp;imaging and quantitative RT-PCR on dorsal root ganglion (DRG) neurons, hematoxylin and eosin staining, immunofluorescence staining of skin preparations, behavioral testing, in combination with genetic and pharmacological approaches, we showed that TRPV1-positive DRG neurons but not mast cells are involved in BB-UVB irradiation-induced itch. Moreover, both genetic and pharmacological inhibition of TRPV1 function significantly alleviated BB-UVB irradiation-induced itch response. Collectively, our results suggest that BB-UVB irradiation evokes itch sensation in mice through promoting TRPV1 channel function in DRG neurons and provide potential therapeutic targets for sunburn-related itch.<\/p>\n\n\n\n<p><strong>Keywords:&nbsp;<\/strong>BB-UVB; Itch model; Sunburn-related itch; TRPV1.<\/p>\n\n\n\n<div class=\"wp-block-file\"><a href=\"http:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2021\/04\/Mechanisms-of-broad-band-ultraviolet-B-irradiation-induced-itch-in-mice-2.pdf\">Mechanisms-of-broad-band-ultraviolet-B-irradiation-induced-itch-in-mice<\/a><a href=\"http:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2021\/04\/Mechanisms-of-broad-band-ultraviolet-B-irradiation-induced-itch-in-mice-2.pdf\" class=\"wp-block-file__button\" download>Download<\/a><\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>J Invest Dermatol.\u00a02021 Apr 1;S0022-202X(21)01129-5.\u00a0doi: 10.1016\/j.jid.2021.03.015.Online ahead of print. Mechanisms of broad-band ultraviolet B irradiation-induced itch in mice Liang Cao\u00a01,\u00a0Xueping Yue\u00a01,\u00a0Yonghui Zhao\u00a01,\u00a0Lixia Du\u00a01,\u00a0Zili Xie\u00a01,\u00a0Yi Yuan\u00a01,\u00a0Sha Zhang\u00a02,\u00a0Feng Li\u00a02,\u00a0Jing Feng\u00a03,\u00a0Hongzhen Hu\u00a01 PMID:&nbsp;33812858 DOI:&nbsp;10.1016\/j.jid.2021.03.015 Abstract Although sunburn can produce severe uncontrollable itching, the underlying mechanisms of ultraviolet (UV) irradiation-induced itch are poorly understood because of a lack of [&hellip;]<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/2246"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2246"}],"version-history":[{"count":2,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/2246\/revisions"}],"predecessor-version":[{"id":2250,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/2246\/revisions\/2250"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2246"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2246"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2246"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}