{"id":2327,"date":"2021-07-29T18:43:22","date_gmt":"2021-07-29T09:43:22","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=2327"},"modified":"2021-07-29T18:43:24","modified_gmt":"2021-07-29T09:43:24","slug":"journal-club-2021-07-30","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=2327","title":{"rendered":"Journal Club-2021.07.30"},"content":{"rendered":"\n

P17 induces chemotaxis and<\/strong> differentiation of monocytes via MRGPRX2-mediated mast cell\u2013line activation<\/strong><\/h2>\n\n\n\n

Journal of allergy and clinical immunology, ~14.11 (IF)<\/p>\n\n\n\n

Karthi Duraisamy, Kailash Singh, Mukesh Kumar, Benjamin Lefranc, Elsa Bonnafe, Michel Treilhou, Jerome Leprince and Billy K. C. Chow, (Hong Kong, China; and Rouen and Albi, France).<\/em><\/p>\n\n\n\n

Abstract<\/strong><\/h2>\n\n\n\n

Background:<\/strong> P17, a peptide isolated from Tetramorium bicarinatum ant venom, is known to induce an alternative phenotype of human monocyte\u2013derived macrophages via activation of an unknown G protein\u2013coupled receptor (GPCR).
<\/p>\n\n\n\n

Objective:<\/strong> We sought to investigate the mechanism of action and the immunomodulatory effects of P17 mediated through MRGPRX2 (Mas related G protein\u2013coupled receptor X2).<\/p>\n\n\n\n

Methods:<\/strong> To identify the GPCR for P17, we screened 314 GPCRs. Upon identification of MRGPRX2, a battery of in silico, in vitro, ex vivo, and in vivo assays along with the receptor mutation studies were performed. In
particular, to investigate the immunomodulatory actions, we used b-hexosaminidase release assay, cytokine releases, quantification of mRNA expression, cell migration and differentiation assays,
immunohistochemical labeling, hematoxylin and eosin, and immunofluorescence staining.
<\/p>\n\n\n\n

Results: <\/strong>P17 activated MRGPRX2 in a dose-dependent manner in b-arrestin recruitment assay. In LAD2 cells, P17 induced calcium and b-hexosaminidase release. Quercetin- and short hairpin RNA\u2013mediated knockdown of MRGPRX2 reduced P17- evoked b-hexosaminidase release. In silico and in vitro mutagenesis studies showed that residue Lys8 of P17 formed a cation-p interaction with the Phe172 of MRGPRX2 and [Ala8
] P17 lost its activity partially. P17 activated LAD2 cells to recruit THP-1 and human monocytes in Transwell migration assay, whereas MRGPRX2-impaired LAD2 cells cannot. In addition, P17-treated LAD2 cells stimulated differentiation of THP-1 and human monocytes, as indicated by the enhanced expression of macrophage markers\u00a0cluster of differentiation\u00a011b and TNF-\u03b1 by quantitative RT-PCR. Immunohistochemical and immunofluorescent staining\u00a0suggested\u00a0monocyte\u00a0recruitment in mice ears injected with P17.<\/p>\n\n\n\n

Conclusion:<\/strong> Our data provide novel structural information regarding the interaction of P17 with MRGPRX2 and intracellular pathways for its immunomodulatory action.<\/p>\n\n\n\n

P17-induces-chemotaxis-and-differentiation-of monocytes via MRGPRX2-mediated mast cell\u2013line activation<\/a>Download<\/a><\/div>\n\n\n\n

Supplementary datas:-<\/strong><\/p>\n\n\n\n

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<\/p>\n","protected":false},"excerpt":{"rendered":"

P17 induces chemotaxis and differentiation of monocytes via MRGPRX2-mediated mast cell\u2013line activation Journal of allergy and clinical immunology, ~14.11 (IF) Karthi Duraisamy, Kailash Singh, Mukesh Kumar, Benjamin Lefranc, Elsa Bonnafe, Michel Treilhou, Jerome Leprince and Billy K. C. Chow, (Hong Kong, China; and Rouen and Albi, France). Abstract Background: P17, a peptide isolated from Tetramorium […]<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/2327"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2327"}],"version-history":[{"count":4,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/2327\/revisions"}],"predecessor-version":[{"id":2349,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/2327\/revisions\/2349"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2327"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2327"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2327"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}