{"id":2540,"date":"2022-06-17T10:40:35","date_gmt":"2022-06-17T01:40:35","guid":{"rendered":"https:\/\/misterx95.myds.me\/?p=2540"},"modified":"2022-06-17T10:40:36","modified_gmt":"2022-06-17T01:40:36","slug":"journal-clun-2022-06-17","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=2540","title":{"rendered":"Journal Clun-2022.06.17"},"content":{"rendered":"\n<h2><strong>A re\u2010innervated\u00a0in vitro\u00a0skin model of non\u2010histaminergic itch and skin neurogenic inflammation: PAR2\u2010, TRPV1\u2010\u00a0and TRPA1\u2010agonist induced functionality<\/strong><\/h2>\n\n\n\n<p><strong>N. Lebonvallet<\/strong>,\u00a0<strong>J. W. Fluhr<\/strong>,\u00a0<strong>C. Le Gall\u2010Ianotto<\/strong>,\u00a0<strong>R. Leschiera<\/strong>, <strong>M. Talagas<\/strong>,\u00a0<strong>J.\u2010L. Carr\u00e9<\/strong>, <strong>A. Reux<\/strong>,\u00a0<strong>L. Misery<\/strong>, <strong>A. Bataille<\/strong>, <strong>C. Brun3<\/strong>, <strong>T. Oddos3<\/strong>, <strong>J.\u2010P. Pennec4<\/strong><\/p>\n\n\n\n<ul><li>Published in:- Skin Health and Disease<\/li><\/ul>\n\n\n\n<h2><strong>Abstract<\/strong><\/h2>\n\n\n\n<p><strong><br>Background:\u00a0<\/strong>Skin, and epidermis, is innervated by sensory nerve fibres. Interactions between them and signal transduction are only partially eluci- dated in physiological\/pathological conditions, especially in pruritus.<\/p>\n\n\n\n<p><strong>Objectives:\u00a0<\/strong>To study the mechanisms involved in pruritus\u00a0in vitro, we developed a skin explant model re\u2010innervated by sensory neurons.<\/p>\n\n\n\n<p><strong>Methods:\u00a0<\/strong>This model is based on the co\u2010culture of human skin explants and sensory neurons from dorsal root ganglia of rats. Innervation and the expression of protease activated receptor 2 (PAR2), transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin one (TRPA1) was analysed by immunostaining. The response of the model to TRPV1, PAR2 and TRPA1 agonists was analysed by patch\u2010clamp, qPCR and enzyme\u2010linked immunosorbent assay.<\/p>\n\n\n\n<p><br><strong>Results:\u00a0<\/strong>After 5 days of re\u2010innervating nerve fibres was evidenced in the epidermis. Re\u2010innervation was correlated with decrease of epidermal thickness and the number of apoptotic cells in the tissue. The major actors of non\u2010histaminergic itch (PAR\u20102, thymic stromal lymphopoietin [TSLP], TSLP\u2010R, TRPA1 and TRPV1) were expressed in neurons and\/or epidermal cells of skin explants. After topical exposure of TRPV1\u2010(Capsaicin), TRPA1\u2010 (Polygodial) and PAR2\u2010agonist (SLIGKV\u2010NH2) activation of reinnervating neurons could be shown in patch\u2010clamp analysis. The release of TSLP was increased with capsaicin or SLIGKV but decreased with polygodial. Release of CGRP was increased by capsaicin and polygodial but decreased with SLIGKV. Activation by SLIGKV showed a decrease of VEGF; polygodial induced an increase of TSLP, Tumour necrosis factor (TNF) and nerve growth factor and capsaicin lead to a decrease of sema3 and TNF expression.<\/p>\n\n\n\n<p><br><strong>Conclusion:\u00a0<\/strong>The present model is suitable for studying itch and neurogenic inflammation pathways\u00a0in vitro. We observed that activation of TRPV1, TRPA1 and PAR\u20102 leads to different response profiles in re\u2010innervated skin explants.<\/p>\n\n\n\n<div class=\"wp-block-file\"><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2022\/06\/SKI2-1-e66-s001.docx\">Supplementary figures<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2022\/06\/SKI2-1-e66-s001.docx\" class=\"wp-block-file__button\" download>Download<\/a><\/div>\n\n\n\n<div class=\"wp-block-file\"><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2022\/06\/SKI2-1-e66-2.pdf\">Article-main<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2022\/06\/SKI2-1-e66-2.pdf\" class=\"wp-block-file__button\" download>Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>A re\u2010innervated\u00a0in vitro\u00a0skin model of non\u2010histaminergic itch and skin neurogenic inflammation: PAR2\u2010, TRPV1\u2010\u00a0and TRPA1\u2010agonist induced functionality N. Lebonvallet,\u00a0J. W. Fluhr,\u00a0C. Le Gall\u2010Ianotto,\u00a0R. Leschiera, M. Talagas,\u00a0J.\u2010L. Carr\u00e9, A. Reux,\u00a0L. Misery, A. Bataille, C. Brun3, T. Oddos3, J.\u2010P. Pennec4 Published in:- Skin Health and Disease Abstract Background:\u00a0Skin, and epidermis, is innervated by sensory nerve fibres. Interactions between [&hellip;]<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/2540"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2540"}],"version-history":[{"count":3,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/2540\/revisions"}],"predecessor-version":[{"id":2545,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/2540\/revisions\/2545"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2540"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2540"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2540"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}