{"id":309,"date":"2013-03-29T10:43:27","date_gmt":"2013-03-29T01:43:27","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=309"},"modified":"2013-04-04T13:01:41","modified_gmt":"2013-04-04T04:01:41","slug":"309","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=309","title":{"rendered":"Journal club 2013-03-29"},"content":{"rendered":"
FASEB J.<\/a>\u00a02012 Dec 27. [Epub ahead of print]<\/div>\n

Superoxide generation and leukocyte accumulation: key elements in the mediation of leukotriene B4-induced itch by transient receptor potential ankyrin 1 and transient receptor potential vanilloid 1.<\/h1>\n
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\r\n\tFilename<\/strong> : fj-12-221218-full.pdf<\/a> (716 KB)\r\n\t
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Fernandes ES<\/a>,\u00a0Vong CT<\/a>,\u00a0Quek S<\/a>,\u00a0Cheong J<\/a>,\u00a0Awal S<\/a>,\u00a0Gentry C<\/a>,\u00a0Aubdool AA<\/a>,\u00a0Liang L<\/a>,\u00a0Bodkin JV<\/a>,\u00a0Bevan S<\/a>,\u00a0Heads R<\/a>,\u00a0Brain SD<\/a>.<\/div>\n
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Source<\/h3>\n

*Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Parasit\u00e1ria, Universidade Ceuma, S\u00e3o Lu\u00eds, Brazil; and \u2020Cardiovascular Division and \u2021Centre for Integrative Biomedicine, British Heart Foundation King’s College London Cardiovascular Centre of Excellence, and \u00a7Wolfson Centre for Age Related Disease, King’s College London, London, UK.<\/p>\n<\/div>\n

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Abstract<\/h3>\n
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The underlying mechanisms of itch are poorly understood. We have investigated a model involving the chemoattractant leukotriene B(4) (LTB(4)) that is up-regulated in common skin diseases. Intradermal injection of LTB(4) (0.1 nmol\/site) into female CD1 mice induced significant scratching movements (used as an itch index) compared with vehicle-injected (0.1% bovine serum albumin-saline) mice. Intraperitoneal transient receptor potential (TRP) channel antagonist treatment significantly inhibited itch as follows: TRP vanilloid 1 (TRPV1) antagonist SB366791 (0.5 mg\/kg, by 97%) and the TRP ankyrin 1 (TRPA1) antagonists TCS 5861528 (10 mg\/kg; 82%) and HC-030031 (100 mg\/kg; 76%). Leukotriene B(4) receptor 2 antagonism by LY255283 (5 mg\/kg i.p.; 62%) reduced itch. Neither TRPV1-knockout (TRPV1-KO) nor TRPA1-knockout (TRPA1-KO mice exhibited LTB(4)-induced itch compared with their wild-type counterparts. The reactive oxygen species scavengers N-acetylcysteine (NAC; 204 mg\/kg i.p.; 86%) or superoxide dismutase (SOD; 10 mg\/kg i.p.; 83%) also inhibited itch. LTB(4)-induced superoxide release was attenuated by TCS 5861528 (56%) and HC-030031 (66%), NAC (58%), SOD (50%), and LY255283 (59%) but not by the leukotriene B(4) receptor 1 antagonist U-75302 (9 nmol\/site) or SB366791. Itch, superoxide, and myeloperoxidase generation were inhibited by the leukocyte migration inhibitor fucoidan (10 mg\/kg i.v.) by 80, 61, and 34%, respectively. Myeloperoxidase activity was also reduced by SB366791 (35%) and SOD (28%). TRPV1-KO mice showed impaired myeloperoxidase release, whereas TRPA1-KO mice exhibited diminished production of superoxide. This result provides novel evidence that TRPA1 and TRPV1 contribute to itch via distinct mechanisms.-Fernandes, E. S., Vong, C. T., Quek, S., Cheong, J., Awal, S., Gentry, C., Aubdool, A. A., Liang, L., Bodkin, J.V., Bevan, S., Heads, R., Brain, S.D. Superoxide generation and leukocyte accumulation: key elements in the mediation of leukotriene B(4)-induced itch by transient receptor potential ankyrin 1 and transient receptor potential vanilloid 1.<\/p>\n

PMID: 23271050<\/span><\/p>\n<\/div>\n<\/div>\n

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FASEB J.\u00a02012 Dec 27. [Epub ahead of print] Superoxide generation and leukocyte accumulation: key elements in the mediation of leukotriene B4-induced itch by transient receptor potential ankyrin 1 and transient receptor potential vanilloid 1. Fernandes ES,\u00a0Vong CT,\u00a0Quek S,\u00a0Cheong J,\u00a0Awal S,\u00a0Gentry C,\u00a0Aubdool AA,\u00a0Liang L,\u00a0Bodkin JV,\u00a0Bevan S,\u00a0Heads R,\u00a0Brain SD. Source *Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Parasit\u00e1ria, Universidade […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/309"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=309"}],"version-history":[{"count":4,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/309\/revisions"}],"predecessor-version":[{"id":314,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/309\/revisions\/314"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=309"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=309"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=309"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}