{"id":3229,"date":"2024-07-25T16:32:39","date_gmt":"2024-07-25T07:32:39","guid":{"rendered":"https:\/\/misterx95.myds.me\/wordpress\/?p=3229"},"modified":"2024-07-25T16:32:40","modified_gmt":"2024-07-25T07:32:40","slug":"2024-07-26-journal-club","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=3229","title":{"rendered":"2024-07-26 Journal Club"},"content":{"rendered":"\n<p><strong>LPS exacerbates TRPV4-mediated itch through the intracellular TLR4-PI3K signalling<\/strong><\/p>\n\n\n\n<p><strong>Yanping Hao1,2,3\u00a0<\/strong>|\u00a0<strong>Liyan Wu1,2\u00a0<\/strong>|\u00a0<strong>Yuhui Wang4\u00a0<\/strong>|\u00a0<strong>Dongmei Shan1,2\u00a0<\/strong>|\u00a0<strong>Yifei Liu1\u00a0<\/strong>|\u00a0<strong>Jing Feng1,2\u00a0<\/strong>|\u00a0<strong>Yi Chang3\u00a0<\/strong>|\u00a0<strong>Ting Wang1,2,5,6<\/strong><\/p>\n\n\n\n<p>J Cell Mol Med \u00a02024 Jul;28(13):e18509.\u00a0doi: 10.1111\/jcmm.18509.<\/p>\n\n\n\n<div class=\"wp-block-file\"><object class=\"wp-block-file__embed\" data=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2024\/07\/J-Cellular-Molecular-Medi-2024-Hao-LPS-exacerbates-TRPV4-mediated-itch-through-the-intracellular-TLR4-PI3K-signalling.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of J-Cellular-Molecular-Medi-2024-Hao-LPS-exacerbates-TRPV4-mediated-itch-through-the-intracellular-TLR4-PI3K-signalling.\"><\/object><a id=\"wp-block-file--media-61249fa2-0970-4f9d-be0e-bba8b36847da\" href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2024\/07\/J-Cellular-Molecular-Medi-2024-Hao-LPS-exacerbates-TRPV4-mediated-itch-through-the-intracellular-TLR4-PI3K-signalling.pdf\">J-Cellular-Molecular-Medi-2024-Hao-LPS-exacerbates-TRPV4-mediated-itch-through-the-intracellular-TLR4-PI3K-signalling<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2024\/07\/J-Cellular-Molecular-Medi-2024-Hao-LPS-exacerbates-TRPV4-mediated-itch-through-the-intracellular-TLR4-PI3K-signalling.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-61249fa2-0970-4f9d-be0e-bba8b36847da\">Download<\/a><\/div>\n\n\n\n<ul>\n<li><sup>1<\/sup>Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.<\/li>\n\n\n\n<li><sup>2<\/sup>University of Chinese Academy of Sciences, Beijing, China.<\/li>\n\n\n\n<li><sup>3<\/sup>Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China.<\/li>\n\n\n\n<li><sup>4<\/sup>Department of Anesthesiology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.<\/li>\n\n\n\n<li><sup>5<\/sup>Yunnan Key Laboratory of Gastrodia and Fungi Symbiotic Biology, Zhaotong University, Zhaotong, Yunnan, China.<\/li>\n\n\n\n<li><sup>6<\/sup>Yunnan Engineering Research Center of Green Planting and Processing of Gastrodia, Zhaotong University, Zhaotong, Yunnan, China.<\/li>\n<\/ul>\n\n\n\n<p>PMID:&nbsp;<strong>38957035<\/strong> PMCID:&nbsp;<a rel=\"noreferrer noopener\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/pmc11220342\/\" target=\"_blank\">PMC11220342<\/a> DOI:&nbsp;<a rel=\"noreferrer noopener\" href=\"https:\/\/doi.org\/10.1111\/jcmm.18509\" target=\"_blank\">10.1111\/jcmm.18509<\/a><\/p>\n\n\n\n<h2>Abstract<\/h2>\n\n\n\n<p>Pruritus is often accompanied with bacterial infections, but the underlying mechanism is not fully understood. Although previous studies revealed that lipopolysaccharides (LPS) could directly activate TRPV4 channel and TRPV4 is involved in the generation of both acute itch and chronic itch, whether and how LPS affects TRPV4-mediated itch sensation remains unclear. Here, we showed that LPS-mediated TRPV4 sensitization exacerbated GSK101-induced scratching behaviour in mice. Moreover, this effect was compromised in TLR4-knockout mice, suggesting LPS acted through a TLR4-dependent mechanism. Mechanistically, LPS enhanced GSK101-evoked calcium influx in mouse ear skin cells and HEK293T cells transfected with TRPV4. Further, LPS sensitized TRPV4 channel through the intracellular TLR4-PI3K-AKT signalling. In summary, our study found a modulatory role of LPS in TRPV4 function and highlighted the TLR4-TRPV4 interaction in itch signal amplification.<\/p>\n\n\n\n<p><strong>Keywords:&nbsp;<\/strong>LPS; PI3K; TLR4; TRPV4; itch sensitization.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>LPS exacerbates TRPV4-mediated itch through the intracellular TLR4-PI3K signalling Yanping Hao1,2,3\u00a0|\u00a0Liyan Wu1,2\u00a0|\u00a0Yuhui Wang4\u00a0|\u00a0Dongmei Shan1,2\u00a0|\u00a0Yifei Liu1\u00a0|\u00a0Jing Feng1,2\u00a0|\u00a0Yi Chang3\u00a0|\u00a0Ting Wang1,2,5,6 J Cell Mol Med \u00a02024 Jul;28(13):e18509.\u00a0doi: 10.1111\/jcmm.18509. PMID:&nbsp;38957035 PMCID:&nbsp;PMC11220342 DOI:&nbsp;10.1111\/jcmm.18509 Abstract Pruritus is often accompanied with bacterial infections, but the underlying mechanism is not fully understood. Although previous studies revealed that lipopolysaccharides (LPS) could directly activate TRPV4 [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3229"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3229"}],"version-history":[{"count":1,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3229\/revisions"}],"predecessor-version":[{"id":3231,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3229\/revisions\/3231"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3229"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3229"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3229"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}