{"id":323,"date":"2013-04-12T11:00:52","date_gmt":"2013-04-12T02:00:52","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=323"},"modified":"2013-07-17T01:26:02","modified_gmt":"2013-07-16T16:26:02","slug":"trpa1-mediates-formalin-induced-pain","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=323","title":{"rendered":"Journal club 2013-04-12"},"content":{"rendered":"

13525-1.full<\/a>TRPA1 mediates formalin-induced pain<\/h1>\n
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Colleen R. McNamara<\/a>,*<\/sup> Josh Mandel-Brehm<\/a>,*<\/sup> Diana M. Bautista<\/a>,\u2020<\/sup> Jan Siemens<\/a>,\u2020<\/sup> Kari L. Deranian<\/a>,*<\/sup> Michael Zhao<\/a>,*<\/sup> Neil J. Hayward<\/a>,*<\/sup> Jayhong A. Chong<\/a>,*<\/sup> David Julius<\/a>,\u2020<\/sup>\u2021<\/sup> Magdalene M. Moran<\/a>,*<\/sup>\u2021<\/sup> and Christopher M. Fanger<\/a>*<\/sup><\/div>\n<\/div>\n
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*Hydra Biosciences, Inc., 790 Memorial Drive, Cambridge, MA 02139; and<\/div>\n
\u2020<\/sup>Departments of Physiology and Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143<\/div>\n
\u2021<\/sup>To whom correspondence may be addressed. E-mail: julius@cmp.ucsf.edu<\/a> or ; Email: mmoran@hydrabiosciences.com<\/a><\/div>\n
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Contributed by David Julius, July 5, 2007<\/p>\n

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Author contributions: C.R.M. and J.M.-B. contributed equally to this work; C.R.M., J.M.-B., D.M.B., J.S., N.J.H., D.J., M.M.M., and C.M.F. designed research; C.R.M., J.M.-B., D.M.B., J.S., K.L.D., and M.Z. performed research; C.R.M., J.M.-B., D.M.B., J.S., M.Z., J.A.C., D.J., M.M.M., and C.M.F. analyzed data; and C.R.M., D.M.B., J.S., J.A.C., D.J., M.M.M., and C.M.F. wrote the paper.<\/p>\n<\/div>\n<\/div>\n

Author information \u25ba<\/a> Article notes \u25ba<\/a> Copyright and License information \u25ba<\/a><\/div>\n
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Received June 18, 2007<\/div>\n<\/div>\n
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Copyright<\/a> \u00a9 2007 by The National Academy of Sciences of the USA<\/div>\n
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Freely available online through the PNAS open access option.<\/p>\n<\/div>\n<\/div>\n<\/div>\n

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This article has been cited by<\/a> other articles in PMC.<\/div>\n<\/div>\n<\/div>\n
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Go to:<\/a><\/div>\n

Abstract<\/h2>\n

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The formalin model is widely used for evaluating the effects of analgesic compounds in laboratory animals. Injection of formalin into the hind paw induces a biphasic pain response; the first phase is thought to result from direct activation of primary afferent sensory neurons, whereas the second phase has been proposed to reflect the combined effects of afferent input and central sensitization in the dorsal horn. Here we show that formalin excites sensory neurons by directly activating TRPA1, a cation channel that plays an important role in inflammatory pain. Formalin induced robust calcium influx in cells expressing cloned or native TRPA1 channels, and these responses were attenuated by a previously undescribed TRPA1-selective antagonist. Moreover, sensory neurons from TRPA1-deficient mice lacked formalin sensitivity. At the behavioral level, pharmacologic blockade or genetic ablation of TRPA1 produced marked attenuation of the characteristic flinching, licking, and lifting responses resulting from intraplantar injection of formalin. Our results show that TRPA1 is the principal site of formalin’s pain-producing action in vivo<\/em>, and that activation of this excitatory channel underlies the physiological and behavioral responses associated with this model of pain hypersensitivity.<\/p>\n<\/div>\n

Keywords: <\/strong>analgesia, inflammation, trp channel, formaldehyde<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

13525-1.fullTRPA1 mediates formalin-induced pain Colleen R. McNamara,* Josh Mandel-Brehm,* Diana M. Bautista,\u2020 Jan Siemens,\u2020 Kari L. Deranian,* Michael Zhao,* Neil J. Hayward,* Jayhong A. Chong,* David Julius,\u2020\u2021 Magdalene M. Moran,*\u2021 and Christopher M. Fanger* *Hydra Biosciences, Inc., 790 Memorial Drive, Cambridge, MA 02139; and \u2020Departments of Physiology and Cellular and Molecular Pharmacology, University of California, […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/323"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=323"}],"version-history":[{"count":3,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/323\/revisions"}],"predecessor-version":[{"id":328,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/323\/revisions\/328"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}