{"id":3308,"date":"2024-12-19T13:54:52","date_gmt":"2024-12-19T04:54:52","guid":{"rendered":"https:\/\/misterx95.myds.me\/wordpress\/?p=3308"},"modified":"2024-12-19T13:58:10","modified_gmt":"2024-12-19T04:58:10","slug":"inhibitory-effect-of-daphnetin-on-the-c48-80-induced-pseudo-allergic-reaction","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=3308","title":{"rendered":"Journal club 2024.12.20"},"content":{"rendered":"\n<h2>Inhibitory effect of daphnetin on the C48\/80-induced pseudo-allergic reaction<\/h2>\n\n\n\n<p>Jingyu&nbsp;Zhang&nbsp;<sup>a<\/sup>,&nbsp;Ling&nbsp;Hong&nbsp;<sup>b<\/sup>,&nbsp;Ping&nbsp;Zhang&nbsp;<sup>a<\/sup>,&nbsp;Yanjie&nbsp;Wang&nbsp;<sup>a<\/sup>,&nbsp;Tie&nbsp;Hong&nbsp;<sup>a<\/sup><\/p>\n\n\n\n<p><a href=\"https:\/\/doi.org\/10.1016\/j.intimp.2023.110874\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.1016\/j.intimp.2023.110874<\/a><a href=\"https:\/\/s100.copyright.com\/AppDispatchServlet?publisherName=ELS&amp;contentID=S1567576923011992&amp;orderBeanReset=true\" target=\"_blank\" rel=\"noreferrer noopener\">Get rights and content<\/a><\/p>\n\n\n\n<h2>Highlights<\/h2>\n\n\n\n<ul>\n<li>Daphnetin&nbsp;inhibits inflammatory mediator release by C48\/80-activated RBL-2H3 cells.<\/li>\n\n\n\n<li>Daphnetin inhibits pseudo-allergic reactions induced by C48\/80 in mouse models.<\/li>\n\n\n\n<li>Daphnetin inhibits C48\/80 induced mast cell activation by inhibiting phosphorylation of PLC\u03b3, IP3R, PKC, ERK1\/2 and P38.<\/li>\n<\/ul>\n\n\n\n<h2>Abstract<\/h2>\n\n\n\n<p>Pseudo-allergic reaction is an allergic reaction mediated by nonimmunoglobulin E (IgE), which does not require prior contact with antigen sensitization and directly leads to mast cell degranulation. Daphnetin (DAP) is known for its anti-inflammatory effects, but there are few studies on the effect of DAP on pseudo-allergy and its mechanism. To investigate the effect of DAP on pseudo-allergy and its mechanism, we inflicted pseudo-allergy on RBL-2H3 cells using C48\/80 in vitro. Moreover, to assess the antipseudo-allergy effect of C48\/80 in vivo, mouse models of local anaphylaxis, systemic anaphylaxis, and itch were used. The in vitro results show that DAP inhibits degranulation and chemokine release; furthermore, DAP reduced the activation of the PLC-IP3R and MAPK signaling pathways induced by C48\/80. Additionally, our in vivo results showed that DAP inhibited C48\/80-induced local anaphylaxis and inhibited eosinophil aggregation, vasodilation and mast cell degranulation. In systemic anaphylaxis, DAP inhibits the decrease in body temperature and reduces the release of His, TNF-a and IL-8. In C48\/80-induced itch, the number of scratches in mice was reduced. Our results demonstrate that DAP can play a suppressive role in the pseudo-allergy induced by C48\/80, providing information for the cure of disorders linked to pseudo-allergic reactions.<\/p>\n\n\n\n<p>Keywords: Pseudo-allergic reaction, Daphnetin,C48\/80,RBL-2H3 cells,PLC\/IP3R<\/p>\n\n\n\n<div class=\"wp-block-file\"><object class=\"wp-block-file__embed\" data=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2024\/12\/Inhibitory-effect-of-daphnetin-on-the-C4880-induced-pseudo-allergic-reaction.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of Inhibitory-effect-of-daphnetin-on-the-C4880-induced-pseudo-allergic-reaction.\"><\/object><a id=\"wp-block-file--media-1906ef45-c7f5-431e-be9c-20dc393725c3\" href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2024\/12\/Inhibitory-effect-of-daphnetin-on-the-C4880-induced-pseudo-allergic-reaction.pdf\">Inhibitory-effect-of-daphnetin-on-the-C4880-induced-pseudo-allergic-reaction<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2024\/12\/Inhibitory-effect-of-daphnetin-on-the-C4880-induced-pseudo-allergic-reaction.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-1906ef45-c7f5-431e-be9c-20dc393725c3\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Inhibitory effect of daphnetin on the C48\/80-induced pseudo-allergic reaction Jingyu&nbsp;Zhang&nbsp;a,&nbsp;Ling&nbsp;Hong&nbsp;b,&nbsp;Ping&nbsp;Zhang&nbsp;a,&nbsp;Yanjie&nbsp;Wang&nbsp;a,&nbsp;Tie&nbsp;Hong&nbsp;a https:\/\/doi.org\/10.1016\/j.intimp.2023.110874Get rights and content Highlights Abstract Pseudo-allergic reaction is an allergic reaction mediated by nonimmunoglobulin E (IgE), which does not require prior contact with antigen sensitization and directly leads to mast cell degranulation. Daphnetin (DAP) is known for its anti-inflammatory effects, but there are few [&hellip;]<\/p>\n","protected":false},"author":20,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3308"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3308"}],"version-history":[{"count":4,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3308\/revisions"}],"predecessor-version":[{"id":3315,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3308\/revisions\/3315"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3308"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3308"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3308"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}