{"id":332,"date":"2013-04-24T17:03:30","date_gmt":"2013-04-24T08:03:30","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=332"},"modified":"2013-04-24T17:05:13","modified_gmt":"2013-04-24T08:05:13","slug":"journal-club-2013-04-26","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=332","title":{"rendered":"Journal club 2013-04-26"},"content":{"rendered":"
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\r\n\t\r\n\t\t\"J.\r\n\t<\/a>\r\n<\/dt>\r\n
\r\n\tFilename<\/strong> : j-biol-chem-2013-than-jbc-m113-450072.pdf<\/a> (1 MB)\r\n\t
\r\n\tCaption<\/strong> : \r\n<\/dd>\r\n<\/dl>

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J Biol Chem.<\/a>\u00a02013 Mar 18. [Epub ahead of print]<\/div>\n

The excitation and modulation of TRPV1-, TRPA1-and TRPM8-expressing sensory neurons by the pruritogen chloroquine.<\/strong><\/p>\n

Than JY<\/a>,\u00a0Li L<\/a>,\u00a0Hasan R<\/a>,\u00a0Zhang X<\/a>.<\/div>\n
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Abstract<\/span><\/h3>\n<\/div>\n
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The sensations of pain,\u00a0itch\u00a0and cold often interact with each other. Pain inhibits\u00a0itch, whereas cold inhibits both pain and\u00a0itch. TRPV1 and TRPA1 channels transduce pain and\u00a0itch, whereas TRPM8 transduces cold. The pruritogen chloroquine (CQ) was reported to excite TRPA1, leading to the sensation of\u00a0itch. It is unclear how CQ excites and modulates TRPA1+, TRPV1+ and TRPM8+ neurons and thus affects the sensations of pain,\u00a0itchand cold. Here, we show that only 43% of CQ-excited dorsal root ganglion (DRG) neurons express TRPA1; as expected, the responses of these neurons were completely prevented by the TRPA1 antagonist HC-030031. The remaining 57% of CQ-excited neurons did not express TRPA1, and excitation was not prevented by either a TRPA1 or a TRPV1 antagonist, but was prevented by the general TRPC channel blocker BTP2 and the selective\u00a0TRPC3\u00a0inhibitor Pyr3. Furthermore, CQ caused potent sensitization of TRPV1 in 51.9% of TRPV1+ neurons, and concomitant inhibition of TRPM8 in 48.8% of TRPM8+ DRG neurons. Sensitization of TRPV1 is mainly caused by activation of the PLC-PKC pathway following activation of the CQ receptor MrgprA3. By contrast, inhibition of TRPM8 is caused by a direct action of activated Galpha q independently of the PLC pathway. Our data suggest the involvement of\u00a0TRPC3\u00a0channel acting together with TRPA1 to mediate CQ-induced\u00a0itch. CQ not only elicits\u00a0itch\u00a0by directly excitingitch-encoding neurons, but also exerts previously unappreciated widespread actions on pain-,\u00a0itch- and cold-sensing neurons, leading to enhanced pain and\u00a0itch.<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

J Biol Chem.\u00a02013 Mar 18. [Epub ahead of print] The excitation and modulation of TRPV1-, TRPA1-and TRPM8-expressing sensory neurons by the pruritogen chloroquine. Than JY,\u00a0Li L,\u00a0Hasan R,\u00a0Zhang X. Abstract The sensations of pain,\u00a0itch\u00a0and cold often interact with each other. Pain inhibits\u00a0itch, whereas cold inhibits both pain and\u00a0itch. TRPV1 and TRPA1 channels transduce pain and\u00a0itch, whereas […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/332"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=332"}],"version-history":[{"count":4,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/332\/revisions"}],"predecessor-version":[{"id":337,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/332\/revisions\/337"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=332"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=332"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=332"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}