{"id":3367,"date":"2025-02-21T03:12:00","date_gmt":"2025-02-20T18:12:00","guid":{"rendered":"https:\/\/misterx95.myds.me\/wordpress\/?p=3367"},"modified":"2025-02-21T07:55:41","modified_gmt":"2025-02-20T22:55:41","slug":"journal-club-2025-02-20","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=3367","title":{"rendered":"Journal club 2025.02.21"},"content":{"rendered":"\n<h2>MrgprX2 regulates mast cell degranulation through PI3K\/AKT and PLC\u03b3 signaling in pseudo-allergic reactions<br><\/h2>\n\n\n\n<p><a rel=\"noreferrer noopener\" href=\"https:\/\/doi.org\/10.1016\/j.intimp.2021.108389\" target=\"_blank\">https:\/\/doi.org\/10.1016\/j.intimp.2021.108389<\/a><br><br>Fan&nbsp;Zhang&nbsp;<sup>ab<\/sup>,&nbsp;Fang&nbsp;Hong&nbsp;<sup>ab<\/sup>,&nbsp;Lu&nbsp;Wang&nbsp;<sup>ac<\/sup>,&nbsp;Renjie&nbsp;Fu&nbsp;<sup>a<\/sup>,&nbsp;Jin&nbsp;Qi&nbsp;<sup>a<\/sup>,&nbsp;Boyang Yu<sup>ab<\/sup><\/p>\n\n\n\n<p><sup>a<\/sup>Jiangsu Key Laboratory for TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 21198, China<br><sup>b<\/sup>Research Center for Traceability and Standardization of TCMs, China Pharmaceutical University, Nanjing 211198, China<br><sup>c<\/sup>Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, China<br><br>Received 10 August 2021, Revised 27 October 2021, Accepted 9 November 2021, Available online 15 December 2021, Version of Record 15 December 2021.<\/p>\n\n\n\n<h2>Highlights<\/h2>\n\n\n\n<ul>\n<li>\u2022MrgprX2 is a key receptor for pseudo-allergic reactions.<\/li>\n\n\n\n<li>\u2022PI3K\/AKT&nbsp;<a href=\"https:\/\/www.sciencedirect.com\/topics\/biochemistry-genetics-and-molecular-biology\/signal-transduction\">signaling pathway<\/a>&nbsp;and PLC\u03b3 are important downstream of MrgprX2.<\/li>\n\n\n\n<li>\u2022Inhibiting PI3K\/AKT signaling pathway and PLC\u03b3 remarkable weakened pseudo-allergic reactions both&nbsp;<em>in vivo<\/em>&nbsp;and&nbsp;<em>in vitro<\/em>.<\/li>\n<\/ul>\n\n\n\n<h2>Abstract<\/h2>\n\n\n\n<p>The G protein-coupled receptor MrgprX2 in mast cells is known to be a crucial receptor for pseudo-allergic reactions. MrgprX2 activation leads to elevated intracellular calcium levels and mast cell degranulation, but the underlying mechanism remains to be elucidated. Herein, we investigated the role of the phosphatidylinositol 3 kinase (PI3K)\/serum-threonine kinase (AKT) signaling pathway and phospholipase C gamma (PLC\u03b3) in mast cell degranulation mediated by MrgprX2 in LAD2 human-derived mast cells. The results showed that phosphorylated AKT (p-AKT) and PLC\u03b3 up-regulation were accompanied by an increase in intracellular calcium following activation of MrgprX2 by Compound 48\/80, an inducer of mast cell degranulation. In contrast, p-AKT and PLC\u03b3 were down-regulated and intracellular calcium levels decreased after MrgprX2 knockdown. Mast cell degranulation was clearly suppressed; however, inhibiting&nbsp;<a href=\"https:\/\/www.sciencedirect.com\/topics\/biochemistry-genetics-and-molecular-biology\/phosphoinositide-3-kinase\">PI3K<\/a>and PLC\u03b3 phosphorylation did not influence MrgprX2 expression. The increase in calcium concentration was suppressed and mast cell degranulation was weakened. Furthermore, by inhibiting&nbsp;<a href=\"https:\/\/www.sciencedirect.com\/topics\/pharmacology-toxicology-and-pharmaceutical-science\/phosphatidylinositol-3-kinase\">PI3K<\/a>&nbsp;and PLC\u03b3 phosphorylation in animals, the allergic symptoms caused by C48\/80 were obviously reduced. We deduced that during the mast cell degranulation observed in pseudoallergic reactions, MrgprX2 regulated intracellular calcium levels via the PI3K\/AKT and PLC\u03b3 pathways.<br><br><\/p>\n\n\n\n<h2>Keywords<\/h2>\n\n\n\n<p>Pseudo-allergic reactions, Mast cell, MRGPRX2, PI3K\/AKT, PLC\u03b3<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<div class=\"wp-block-file\"><object class=\"wp-block-file__embed\" data=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2025\/02\/MrgprX2-regulates-mast-cell-degranulation-through-PI3KAKT-and-PLC\u03b3-signaling-in-pseudo-allergic-reactions.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of MrgprX2-regulates-mast-cell-degranulation-through-PI3KAKT-and-PLC\u03b3-signaling-in-pseudo-allergic-reactions.\"><\/object><a id=\"wp-block-file--media-72e0a593-2f05-4903-a4b0-c0ca08560221\" href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2025\/02\/MrgprX2-regulates-mast-cell-degranulation-through-PI3KAKT-and-PLC\u03b3-signaling-in-pseudo-allergic-reactions.pdf\">MrgprX2-regulates-mast-cell-degranulation-through-PI3KAKT-and-PLC\u03b3-signaling-in-pseudo-allergic-reactions<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2025\/02\/MrgprX2-regulates-mast-cell-degranulation-through-PI3KAKT-and-PLC\u03b3-signaling-in-pseudo-allergic-reactions.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-72e0a593-2f05-4903-a4b0-c0ca08560221\">Download<\/a><\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>MrgprX2 regulates mast cell degranulation through PI3K\/AKT and PLC\u03b3 signaling in pseudo-allergic reactions https:\/\/doi.org\/10.1016\/j.intimp.2021.108389 Fan&nbsp;Zhang&nbsp;ab,&nbsp;Fang&nbsp;Hong&nbsp;ab,&nbsp;Lu&nbsp;Wang&nbsp;ac,&nbsp;Renjie&nbsp;Fu&nbsp;a,&nbsp;Jin&nbsp;Qi&nbsp;a,&nbsp;Boyang Yuab aJiangsu Key Laboratory for TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 21198, ChinabResearch Center for Traceability and Standardization of TCMs, China Pharmaceutical University, Nanjing 211198, ChinacNanjing Hospital of Chinese Medicine Affiliated to [&hellip;]<\/p>\n","protected":false},"author":20,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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