{"id":339,"date":"2013-04-29T19:16:18","date_gmt":"2013-04-29T10:16:18","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=339"},"modified":"2013-04-29T19:16:18","modified_gmt":"2013-04-29T10:16:18","slug":"journal-club-2013-04-30","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=339","title":{"rendered":"Journal club 2013-04-30"},"content":{"rendered":"
\r\n
\r\n\t\r\n\t\t\"2383.full\"\r\n\t<\/a>\r\n<\/dt>\r\n
\r\n\tFilename<\/strong> : 2383-full.pdf<\/a> (969 KB)\r\n\t
\r\n\tCaption<\/strong> : \r\n<\/dd>\r\n<\/dl>

<\/p><\/div><\/div>\n

Proc Natl Acad Sci U S A.<\/a>\u00a02013 Feb 5;110(6):2383-8. doi: 10.1073\/pnas.1215555110. Epub 2013 Jan 23.<\/div>\n

UV\u00a0light\u00a0phototransduction\u00a0activates\u00a0transient\u00a0receptor\u00a0potential\u00a0A1\u00a0ion\u00a0channels\u00a0in\u00a0human\u00a0melanocytes.<\/h1>\n
Bellono NW<\/a>,\u00a0Kammel LG<\/a>,\u00a0Zimmerman AL<\/a>,\u00a0Oancea E<\/a>.<\/div>\n
\n

Source<\/h3>\n

Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI 02912, USA.<\/p>\n<\/div>\n

\n

Abstract<\/h3>\n
\n

Human\u00a0skin is constantly exposed to solar ultraviolet radiation (UVR), the most prevalent environmental carcinogen. Humans have the unique ability among mammals to respond to UVR by increasing their skin pigmentation, a protective process driven by melanin synthesis in epidermalmelanocytes. The molecular mechanisms used by\u00a0melanocytes\u00a0to detect and respond to long-wavelength UVR (UVA) are not well understood. We recently identified a UVA\u00a0phototransduction\u00a0pathway in\u00a0melanocytes\u00a0that is mediated by G protein-coupled receptors and leads to rapid calcium mobilization. Here we report that in\u00a0human\u00a0epidermal\u00a0melanocytes\u00a0physiological doses of UVR activate a retinal-dependent current mediated bytransient\u00a0receptor\u00a0potential\u00a0A1\u00a0(TRPA1)\u00a0ion\u00a0channels. The TRPA1 photocurrent is UVA-specific and requires G protein and phospholipase C signaling, thus contributing to UVA-induced calcium responses to mediate downstream cellular effects and providing evidence for TRPA1 function in mammalianphototransduction. Remarkably, TRPA1 activation is required for the UVR-induced and retinal-dependent early increase in cellular melanin. Our results show that TRPA1 is essential for a unique extraocular\u00a0phototransduction\u00a0pathway in\u00a0human\u00a0melanocytes\u00a0that is activated by physiological doses of UVR and results in early melanin synthesis.<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Proc Natl Acad Sci U S A.\u00a02013 Feb 5;110(6):2383-8. doi: 10.1073\/pnas.1215555110. Epub 2013 Jan 23. UV\u00a0light\u00a0phototransduction\u00a0activates\u00a0transient\u00a0receptor\u00a0potential\u00a0A1\u00a0ion\u00a0channels\u00a0in\u00a0human\u00a0melanocytes. Bellono NW,\u00a0Kammel LG,\u00a0Zimmerman AL,\u00a0Oancea E. Source Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI 02912, USA. Abstract Human\u00a0skin is constantly exposed to solar ultraviolet radiation (UVR), the most prevalent environmental carcinogen. Humans have the unique ability […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/339"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=339"}],"version-history":[{"count":1,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/339\/revisions"}],"predecessor-version":[{"id":341,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/339\/revisions\/341"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=339"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=339"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}