{"id":3683,"date":"2026-03-20T09:08:11","date_gmt":"2026-03-20T00:08:11","guid":{"rendered":"https:\/\/misterx95.myds.me\/wordpress\/?p=3683"},"modified":"2026-03-26T14:53:59","modified_gmt":"2026-03-26T05:53:59","slug":"journal-club-26-03-20","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=3683","title":{"rendered":"Journal Club 26.03.20"},"content":{"rendered":"\n<h2 class=\"has-black-color has-text-color\" style=\"font-size:31px\">Tussilagone inhibits MRGPRX2-mediated mast cell degranulation and suppresses pseudo-allergic reactions<\/h2>\n\n\n\n<h2>Highlights<\/h2>\n\n\n\n<ul>\n<li>\u2022Tussilagone attenuates Tween 80 and Substance P-induced pseudoallergic reactions.<\/li>\n\n\n\n<li>\u2022Tussilagone targets the downstream signaling pathway of MRGPRX2.<\/li>\n\n\n\n<li>\u2022Tussilagone suppresses pseudo-allergic reactions via Lyn-Btk-PLC\u03b3-Ca<sup>2+<\/sup>&nbsp;and p38\/NF-\u03baB pathways.<\/li>\n<\/ul>\n\n\n\n<h2>Abstract<\/h2>\n\n\n\n<p>Mas-related G protein-coupled receptor X2 (MRGPRX2) is a crucial target in pseudo-allergic reactions. Tussilagone (Tus), the main bioactive component derived from&nbsp;<em>Tussilago farfara<\/em>, has anti-inflammatory effects, but its potential inhibitory effects on pseudo-allergic responses remain unclear. This research aimed to evaluate the inhibitory role of Tus on pseudo-allergic reactions and its underlying mechanism. In vivo Systemic pseudo-allergic reactions and passive cutaneous anaphylaxis (PCA) models were established to assess the effects of Tus. In vitro, mast cell (LAD2) degranulation, inflammatory cytokine release, and signaling pathway protein expression were assessed. Calcium influx was measured in MRGPRX2-expressing HEK293 cells. The results showed that Tus significantly attenuated Tween 80- and substance P (SP)-induced systemic pseudo-allergy and PCA reactions. It also suppressed mast cell degranulation and decreased production of tumor necrosis factor-alpha (TNF-\u03b1), Interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1). In MRGPRX2-expressing HEK293 cells, Tus suppressed Tween 80- and SP-induced Ca<sup>2+<\/sup>&nbsp;influx. Mechanistically, Tus inhibited tolimidone-induced Lyn kinase activation and suppressed SP-and Tween 80-induced \u03b2-hexosaminidase release, exhibiting an inhibitory profile comparable to that of the Lyn\/Btk antagonist bosutinib. Additionally, Tus attenuated the phosphorylation levels of MRGPRX2 downstream signal molecules, including Btk, PLC\u03b31, PKC, p38 MAPK, I\u03baB-\u03b1 and NF-\u03baB (p65). In conclusion, Tus attenuates SP-and Tween 80-induced mast cell activation and pseudo-allergic reactions by targeting the Lyn\/Btk\/PLC\u03b31 and p38\/NF-\u03baB pathways, highlighting its therapeutic potential for pseudo-allergy.<\/p>\n\n\n\n<h2>Keywords<\/h2>\n\n\n\n<p>pseudo-allergic reactions; Tussilagone; Mast cell; Lyn; MRGPRX2<\/p>\n\n\n\n<ul>\n<li><\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/doi.org\/10.1016\/j.taap.2026.117763\">https:\/\/doi.org\/10.1016\/j.taap.2026.117763<\/a><\/p>\n\n\n\n<ul>\n<li><\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-file\"><object class=\"wp-block-file__embed\" data=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/03\/1-s2.0-S0041008X26000591-main.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of 1-s2.0-S0041008X26000591-main.\"><\/object><a id=\"wp-block-file--media-1bf6d7ba-c15f-43a2-b917-c618f9ef199a\" href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/03\/1-s2.0-S0041008X26000591-main.pdf\">1-s2.0-S0041008X26000591-main<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/03\/1-s2.0-S0041008X26000591-main.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-1bf6d7ba-c15f-43a2-b917-c618f9ef199a\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Tussilagone inhibits MRGPRX2-mediated mast cell degranulation and suppresses pseudo-allergic reactions Highlights Abstract Mas-related G protein-coupled receptor X2 (MRGPRX2) is a crucial target in pseudo-allergic reactions. Tussilagone (Tus), the main bioactive component derived from&nbsp;Tussilago farfara, has anti-inflammatory effects, but its potential inhibitory effects on pseudo-allergic responses remain unclear. This research aimed to evaluate the inhibitory role [&hellip;]<\/p>\n","protected":false},"author":22,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3683"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/22"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3683"}],"version-history":[{"count":4,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3683\/revisions"}],"predecessor-version":[{"id":3702,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3683\/revisions\/3702"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3683"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3683"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3683"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}