{"id":3739,"date":"2026-05-15T10:26:44","date_gmt":"2026-05-15T01:26:44","guid":{"rendered":"https:\/\/misterx95.myds.me\/wordpress\/?p=3739"},"modified":"2026-05-15T10:26:45","modified_gmt":"2026-05-15T01:26:45","slug":"journal-club-26-05-15","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=3739","title":{"rendered":"\u00a0Journal club 26.05.15"},"content":{"rendered":"\n<p>Neuropsin, TRPV4 and intracellular calcium mediate intrinsic photosensitivity in corneal epithelial cells<\/p>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Lapajne+L&amp;cauthor_id=39681161\">Luka Lapajne<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39681161\/#full-view-affiliation-1\">1<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Lakk+M&amp;cauthor_id=39681161\">Monika Lakk<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39681161\/#full-view-affiliation-2\">2<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Rudzitis+CN&amp;cauthor_id=39681161\">Christopher N Rudzitis<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39681161\/#full-view-affiliation-3\">3<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Vemaraju+S&amp;cauthor_id=39681161\">Shruti Vemaraju<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39681161\/#full-view-affiliation-4\">4<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Lang+RA&amp;cauthor_id=39681161\">Richard A Lang<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39681161\/#full-view-affiliation-4\">4<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Hawlina+M&amp;cauthor_id=39681161\">Marko Hawlina<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39681161\/#full-view-affiliation-5\">5<\/a><\/sup>,\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Kri%C5%BEaj+D&amp;cauthor_id=39681161\">David Kri\u017eaj<\/a><sup>\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39681161\/#full-view-affiliation-6\">6<\/a><\/sup><\/p>\n\n\n\n<ul>\n<li><sup>1<\/sup>Department of Ophthalmology &amp; Visual Sciences, University of Utah School of Medicine, Salt Lake City, UT, USA; Department of Ophthalmology, University Medical Center, Ljubljana, Slovenia.<\/li>\n\n\n\n<li><sup>2<\/sup>Department of Ophthalmology &amp; Visual Sciences, University of Utah School of Medicine, Salt Lake City, UT, USA.<\/li>\n\n\n\n<li><sup>3<\/sup>Department of Ophthalmology &amp; Visual Sciences, University of Utah School of Medicine, Salt Lake City, UT, USA; Interdepartmental Program in Neuroscience, University of Utah, USA.<\/li>\n\n\n\n<li><sup>4<\/sup>Department of Ophthalmology, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.<\/li>\n\n\n\n<li><sup>5<\/sup>Department of Ophthalmology, University Medical Center, Ljubljana, Slovenia.<\/li>\n\n\n\n<li><sup>6<\/sup>Department of Ophthalmology &amp; Visual Sciences, University of Utah School of Medicine, Salt Lake City, UT, USA; Interdepartmental Program in Neuroscience, University of Utah, USA; Department of Bioengineering, University of Utah, Salt Lake City, UT, USA; Department of Neurobiology, University of Utah, Salt Lake City, UT, USA. Electronic address: david.krizaj@hsc.utah.edu.<\/li>\n<\/ul>\n\n\n\n<h2>Abstract<\/h2>\n\n\n\n<p><strong>Purpose:&nbsp;<\/strong>To investigate intrinsic phototransduction in the corneal epithelium and its role in intracellular and inflammatory signaling.<\/p>\n\n\n\n<p><strong>Methods:&nbsp;<\/strong>Optical imaging in isolated corneal epithelial cells (CECs) and debrided epithelia was combined with molecular, biochemical, pharmacological assays and gene deletion studies to track UVB-induced calcium signaling and release of cytokines, chemokines and matrix remodeling enzymes. Results from wild type mouse CECs were compared to data obtained from Opn5<sup>-\/-<\/sup>&nbsp;and Trpv4<sup>-\/-<\/sup>&nbsp;cells.<\/p>\n\n\n\n<p><strong>Results:&nbsp;<\/strong>UVB stimuli and TRPV4 activity induced epithelial release of IL-1\u03b2, IL-17, matrix metalloproteinases MMP-3\/MMP-9, and thymic stromal lymphopoietin (TSLP). UVB stimuli evoked [Ca<sup>2+<\/sup>]<sub>i<\/sub>&nbsp;elevations in dissociated mouse CECs that were partially reduced by inhibition of TRPV4 channels, Trpv4 knockdown and replacement of control saline with Ca<sup>2+<\/sup>-free saline. UVB-induced Ca<sup>2+<\/sup>&nbsp;responses were significantly suppressed by OPN5 deletion and by inhibition of phospholipase C signaling, and responses were abrogated in cells with depleted intracellular Ca<sup>2+<\/sup>&nbsp;stores.<\/p>\n\n\n\n<p><strong>Conclusions:&nbsp;<\/strong>Mammalian CECs are intrinsically and constitutively photosensitive. UVB photons are transduced by neuropsin, phospholipase C and CICR signaling, with mouse but not human CE transduction exhibiting a UVB-sensitive TRPV4 component. TRPV4 activity and UVB transduction are linked to cell-autonomous release of proinflammatory, matrix remodeling and nociceptive interleukins and MMPS. TRPV4-induced cytokine release may contribute to the pain induced by mechanical injury of the cornea and CEC photosensing may alert and protect the visual system from ultraviolet B (UVB) radiation -induced snow blindness, injury, vision loss and cancer.<\/p>\n\n\n\n<p><strong>Keywords:&nbsp;<\/strong>Corneal epithelium; Neuropsin; Phototransduction; Snow blindness; TRPV4.<\/p>\n\n\n\n<div class=\"wp-block-file\"><object class=\"wp-block-file__embed\" data=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/1-s2.0-S154201242400137X-main.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of 1-s2.0-S154201242400137X-main.\"><\/object><a id=\"wp-block-file--media-286fd1ec-9ff0-441f-94af-5e1290f0b476\" href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/1-s2.0-S154201242400137X-main.pdf\">1-s2.0-S154201242400137X-main<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/1-s2.0-S154201242400137X-main.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-286fd1ec-9ff0-441f-94af-5e1290f0b476\">Download<\/a><\/div>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-a909402a-34c1-48de-87ee-125c949ac9ed\" href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/1-s2.0-S154201242400137X-mmc1.docx\">1-s2.0-S154201242400137X-mmc1<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/1-s2.0-S154201242400137X-mmc1.docx\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-a909402a-34c1-48de-87ee-125c949ac9ed\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Neuropsin, TRPV4 and intracellular calcium mediate intrinsic photosensitivity in corneal epithelial cells Luka Lapajne\u00a01,\u00a0Monika Lakk\u00a02,\u00a0Christopher N Rudzitis\u00a03,\u00a0Shruti Vemaraju\u00a04,\u00a0Richard A Lang\u00a04,\u00a0Marko Hawlina\u00a05,\u00a0David Kri\u017eaj\u00a06 Abstract Purpose:&nbsp;To investigate intrinsic phototransduction in the corneal epithelium and its role in intracellular and inflammatory signaling. Methods:&nbsp;Optical imaging in isolated corneal epithelial cells (CECs) and debrided epithelia was combined with molecular, biochemical, [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3739"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3739"}],"version-history":[{"count":1,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3739\/revisions"}],"predecessor-version":[{"id":3742,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3739\/revisions\/3742"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3739"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3739"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3739"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}