{"id":3744,"date":"2026-05-28T14:07:57","date_gmt":"2026-05-28T05:07:57","guid":{"rendered":"https:\/\/misterx95.myds.me\/wordpress\/?p=3744"},"modified":"2026-05-28T14:07:58","modified_gmt":"2026-05-28T05:07:58","slug":"journal-club-2026-05-29","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=3744","title":{"rendered":"Journal Club 2026.05.29"},"content":{"rendered":"\n<h1><strong>In silico discovery of nanobody binders to a G protein coupled receptor using AlphaFold Multimer<\/strong><\/h1>\n\n\n\n<p>Edward P. Harvey<sup>1,*<\/sup> , Jeffrey S. Smith<sup>1,2, *<\/sup>, Joseph D. Hurley<sup>1,*<\/sup>, Alyana Granados<sup>3<\/sup>, Ernst W. Schmid<sup>1<\/sup>, Jason G. Liang-Lin<sup>1<\/sup>, Huyang Zhang<sup>1<\/sup>, Emily M. Meara<sup>1,2<\/sup>, Elizabeth K. Wren<sup>1<\/sup>, Steffanie Paul<sup>4,5<\/sup>, Matthew P. Ferguson<sup>1<\/sup>, Victor G. Calvillo-Miranda<sup>1<\/sup>, Miguel A. Alcantar<sup>1,6<\/sup>, Debora S. Marks<sup>4,5<\/sup>, Johannes C. Walter<sup>1,7<\/sup>, Andrew C. Kruse<sup>1,\u2020<\/sup>, Katherine J. Susa<sup>3,\u2020<\/sup><\/p>\n\n\n\n<p><sup>1<\/sup>Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.<br><sup>2<\/sup>Department of Dermatology, Brigham and Women\u2019s Hospital, Boston, MA, 02115,USA.<br><sup>3<\/sup>Department of Pharmaceutical Chemistry, University of California, San Francisco, CA94158, USA<br><sup>4<\/sup>Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA<br><sup>5<\/sup>Broad Institute of Harvard and MIT, Cambridge, MA, 02142, USA<br><sup>6<\/sup>Department of Biomedical Engineering, University of California, Irvine, CA, 92697, USA<br><sup>7<\/sup>Howard Hughes Medical Institute, Boston, MA, USA<br><sup>*<\/sup>These authors contributed equally.<br><sup>\u2020<\/sup>Correspondence to: Andrew C. Kruse (Andrew_kruse@hms.harvard.edu) and Katherine J. Susa (Katherine.Susa@ucsf.edu)<\/p>\n\n\n\n<ul>\n<li>Received02 October 2025<\/li>\n\n\n\n<li>Accepted03 April 2026<\/li>\n\n\n\n<li>Published23 April 2026<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/doi.org\/10.1038\/s41467-026-72093-5\">https:\/\/doi.org\/10.1038\/s41467-026-72093-5<\/a><\/p>\n\n\n\n<div class=\"wp-block-file\"><object class=\"wp-block-file__embed\" data=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.\"><\/object><a id=\"wp-block-file--media-c1b33bf6-9848-42a2-93a3-be583d3243b2\" href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.pdf\">In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-c1b33bf6-9848-42a2-93a3-be583d3243b2\">Download<\/a><\/div>\n\n\n\n<div class=\"wp-block-file\"><object class=\"wp-block-file__embed\" data=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/Supplementary-data_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of Supplementary-data_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.\"><\/object><a id=\"wp-block-file--media-706fc6c9-fba6-4b93-8735-9d17a73c29c2\" href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/Supplementary-data_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.pdf\">Supplementary-data_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/Supplementary-data_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-706fc6c9-fba6-4b93-8735-9d17a73c29c2\">Download<\/a><\/div>\n\n\n\n<div class=\"wp-block-file\"><object class=\"wp-block-file__embed\" data=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/Peer-review-file_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of Peer-review-file_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.\"><\/object><a id=\"wp-block-file--media-2689db60-e083-4714-a8dc-918c415abe00\" href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/Peer-review-file_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.pdf\">Peer-review-file_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer<\/a><a href=\"https:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2026\/05\/Peer-review-file_In-silico-discovery-of-nanobody-binders-to-a-G-protein-coupled-receptor-using-AlphaFold-Multimer.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-2689db60-e083-4714-a8dc-918c415abe00\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>In silico discovery of nanobody binders to a G protein coupled receptor using AlphaFold Multimer Edward P. Harvey1,* , Jeffrey S. Smith1,2, *, Joseph D. Hurley1,*, Alyana Granados3, Ernst W. Schmid1, Jason G. Liang-Lin1, Huyang Zhang1, Emily M. Meara1,2, Elizabeth K. Wren1, Steffanie Paul4,5, Matthew P. Ferguson1, Victor G. Calvillo-Miranda1, Miguel A. Alcantar1,6, Debora S. [&hellip;]<\/p>\n","protected":false},"author":14,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3744"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/14"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3744"}],"version-history":[{"count":1,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3744\/revisions"}],"predecessor-version":[{"id":3748,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/3744\/revisions\/3748"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3744"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3744"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3744"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}