{"id":379,"date":"2013-07-12T10:31:16","date_gmt":"2013-07-12T01:31:16","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=379"},"modified":"2013-07-17T01:30:24","modified_gmt":"2013-07-16T16:30:24","slug":"journal-club-20130712","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=379","title":{"rendered":"Journal club 2013-07-12"},"content":{"rendered":"<p><strong>Lysophosphatidic acid directly activates TRPV1\u00a0through a C-terminal binding site<\/strong><\/p>\n<p>Andr\u00e9s Nieto-Posadas1, Giovanni Picazo-Ju\u00e1rez1, Itzel Llorente1, Andr\u00e9s Jara-Oseguera2,<br \/>\nSara Morales-L\u00e1zaro1, Diana Escalante-Alcalde1*, Le\u00f3n D Islas2* &amp; Tamara Rosenbaum1*<\/p>\n<p>Since 1992, there has been growing evidence that the bioactive phospholipid lysophosphatidic acid (LPA), whose amounts are<br \/>\nincreased upon tissue injury, activates primary nociceptors resulting in neuropathic pain. The TRPV1 ion channel is expressed in<br \/>\nprimary afferent nociceptors and is activated by physical and chemical stimuli. Here we show that in control mice LPA produces<br \/>\nacute pain-like behaviors, which are substantially reduced in Trpv1-null animals. Our data also demonstrate that LPA activates<br \/>\nTRPV1 through a unique mechanism that is independent of G protein\u2013coupled receptors, contrary to what has been widely<br \/>\nshown for other ion channels, by directly interacting with the C terminus of the channel. We conclude that TRPV1 is a direct<br \/>\nmolecular target of the pain-producing molecule LPA and that this constitutes, to our knowledge, the first example of LPA<br \/>\nbinding directly to an ion channel to acutely regulate its function.<\/p>\n<p><a href=\"http:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2013\/07\/nchembio.712.pdf\">nchembio.712<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Lysophosphatidic acid directly activates TRPV1\u00a0through a C-terminal binding site Andr\u00e9s Nieto-Posadas1, Giovanni Picazo-Ju\u00e1rez1, Itzel Llorente1, Andr\u00e9s Jara-Oseguera2, Sara Morales-L\u00e1zaro1, Diana Escalante-Alcalde1*, Le\u00f3n D Islas2* &amp; Tamara Rosenbaum1* Since 1992, there has been growing evidence that the bioactive phospholipid lysophosphatidic acid (LPA), whose amounts are increased upon tissue injury, activates primary nociceptors resulting in neuropathic pain. [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/379"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=379"}],"version-history":[{"count":4,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/379\/revisions"}],"predecessor-version":[{"id":382,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/379\/revisions\/382"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=379"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=379"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=379"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}