{"id":399,"date":"2013-07-19T10:16:38","date_gmt":"2013-07-19T01:16:38","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=399"},"modified":"2013-07-19T10:16:38","modified_gmt":"2013-07-19T01:16:38","slug":"journal-club-2013-07-19","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=399","title":{"rendered":"Journal club 2013-07-19"},"content":{"rendered":"<div><a href=\"http:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2013\/07\/1-s2.0-S0024320506006515-main.pdf\">1-s2.0-S0024320506006515-main<\/a><\/div>\n<div><a title=\"Life sciences.\" role=\"menuitem\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/16979191#\">Life Sci.<\/a>\u00a02006 Dec 14;80(2):89-97. Epub 2006 Aug 23.<\/div>\n<h1>Characterization of the transient receptor potential channels mediating lysophosphatidic acid-stimulated calcium mobilization in B lymphoblasts.<\/h1>\n<div><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed?term=Roedding%20AS%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=16979191\">Roedding AS<\/a>,\u00a0<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed?term=Li%20PP%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=16979191\">Li PP<\/a>,\u00a0<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed?term=Warsh%20JJ%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=16979191\">Warsh JJ<\/a>.<\/div>\n<div>\n<h3>Source<\/h3>\n<p>Laboratory of Cellular and Molecular Pathophysiology, Centre for Addiction and Mental Health, Toronto, ON, Canada M5T 1R8.<\/p>\n<\/div>\n<div>\n<h3>Abstract<\/h3>\n<div>\n<p>Altered 1-oleoyl-lysophosphatidic acid (LPA, 100 microM)-stimulated calcium responses occur in B-lymphoblast cell lines from bipolar disorder patients, but the mechanism(s) involved is uncertain. Lysophosphatidic acid shares a structurally similar fatty acid side chain with the diacylglycerol analogue, 1-oleoyl-2-acetyl-sn-glycerol (OAG), a known activator of subtypes 3, 6 and 7 of the canonical transient receptor potential (TRPC) cation channel subfamily. Accordingly, the objective of this study was to determine whether the LPA-stimulated calcium response in B-lymphoblasts is mediated, in part, through this TRPC channel subfamily. Divalent cation selectivity in response to thapsigargin, LPA and OAG were used to distinguish TRPC-like character of the responses to these agents in BLCLs. The sensitivity to gadolinium, an inhibitor of capacitative calcium channels, was used to determine the store-operated nature of the responses. The TRPC isoforms that are present in BLCLs as identified by immunoblotting and\/or PCR include TRPC1, 3 and 5. Minimal barium influx in calcium-free buffer was observed following thapsigargin stimulation. However, LPA stimulated barium influx of a magnitude similar to that induced by OAG. Thapsigargin-provoked calcium influx was completely inhibited by gadolinium (10 microM), whereas LPA and OAG-stimulated responses were partially inhibited and potentiated, respectively. The results suggest that 100 microM LPA stimulates calcium entry through channels with characteristics similar to TRPC3, as TRPC6 and 7 are absent in B-lymphoblasts.<\/p>\n<\/div>\n<\/div>\n<div>\n<div>\n<dl>\n<dt>PMID:<\/dt>\n<p>\u00a0<\/p>\n<dd>16979191<\/dd>\n<p>\u00a0<\/p>\n<dd>[PubMed &#8211; indexed for MEDLINE]<\/dd>\n<\/dl>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>1-s2.0-S0024320506006515-main Life Sci.\u00a02006 Dec 14;80(2):89-97. Epub 2006 Aug 23. Characterization of the transient receptor potential channels mediating lysophosphatidic acid-stimulated calcium mobilization in B lymphoblasts. Roedding AS,\u00a0Li PP,\u00a0Warsh JJ. Source Laboratory of Cellular and Molecular Pathophysiology, Centre for Addiction and Mental Health, Toronto, ON, Canada M5T 1R8. Abstract Altered 1-oleoyl-lysophosphatidic acid (LPA, 100 microM)-stimulated calcium responses [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/399"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=399"}],"version-history":[{"count":2,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/399\/revisions"}],"predecessor-version":[{"id":402,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/399\/revisions\/402"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=399"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=399"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=399"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}