{"id":92,"date":"2012-08-22T19:05:39","date_gmt":"2012-08-22T10:05:39","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=92"},"modified":"2012-11-27T14:09:34","modified_gmt":"2012-11-27T05:09:34","slug":"journal-club-2012-08-22","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=92","title":{"rendered":"Journal club 2012-08-22"},"content":{"rendered":"<h1><a href=\"http:\/\/misterx95.myds.me\/wordpress\/wp-content\/uploads\/2012\/08\/1-s2.0-S0304394004000370-main.pdf\">1-s2.0-S0304394004000370-main<\/a><\/h1>\n<h1>Histamine-induced Ca<sup>2+<\/sup>influx via the PLA<sub>2<\/sub>\/lipoxygenase\/TRPV1 pathway in rat sensory neurons <sup><a id=\"bitem1\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0304394004000370#item1\">\u2606<\/a><\/sup><\/h1>\n<div>This paper is in honor of Manfred Zimmermann&#8217;s 70th birthday.<\/div>\n<ul>\n<li>Byung Moon Kim,<\/li>\n<li>Sang Hee Lee,<\/li>\n<li>Won Sik Shim,<\/li>\n<li>Uhtaek Oh<a id=\"bCOR1\" title=\"Corresponding author contact information\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0304394004000370#COR1\"><img decoding=\"async\" src=\"http:\/\/cdn.els-cdn.com\/sd\/entities\/REcor.gif\" alt=\"Corresponding author contact information\" \/><\/a><sup>, <\/sup><a href=\"mailto:utoh@plaza.snu.ac.kr\"><img decoding=\"async\" src=\"http:\/\/cdn.els-cdn.com\/sd\/entities\/REemail.gif\" alt=\"E-mail the corresponding author\" \/><\/a><\/li>\n<\/ul>\n<ul>\n<li>Sensory Research Center, National Creative Research Initiatives, College of Pharmacy, Seoul National University, San 56-1, Shinlim, Kwanak-Gu, Seoul 151-742, South Korea<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li>Available online 27 March 2004.<\/li>\n<\/ul>\n<ul>\n<li><a id=\"ddDoi\" href=\"http:\/\/dx.doi.org\/10.1016\/j.neulet.2004.01.019\" target=\"doilink\">http:\/\/dx.doi.org\/10.1016\/j.neulet.2004.01.019<\/a>, <a href=\"http:\/\/www.sciencedirect.com\/science\/help\/doi.htm\" target=\"sdhelp\">How to Cite or Link Using DOI<\/a><\/li>\n<li>\n<div><a href=\"http:\/\/www.sciencedirect.com\/science?_ob=RedirectURL&amp;_method=outwardLink&amp;_partnerName=656&amp;_eid=1-s2.0-S0304394004000370&amp;_pii=S0304394004000370&amp;_origin=article&amp;_zone=art_page&amp;_targetURL=http%3A%2F%2Fwww.scopus.com%2Finward%2Fcitedby.url%3Feid%3D2-s2.0-2342466078%26partnerID%3D10%26rel%3DR3.0.0%26md5%3D01592fae171650131905f0b1d587a85f&amp;_acct=C000057889&amp;_version=1&amp;_userid=8842358&amp;md5=3dc4b0cfdfff411a70f0950f1be66b3e\" rel=\"nofollow\" target=\"outwardLink\">Cited by in Scopus (45)<\/a><\/div>\n<\/li>\n<\/ul>\n<ul>\n<li>\n<div><a href=\"http:\/\/www.sciencedirect.com\/science?_ob=RedirectURL&amp;_method=outwardLink&amp;_partnerName=936&amp;_eid=1-s2.0-S0304394004000370&amp;_pii=S0304394004000370&amp;_origin=article&amp;_zone=art_page&amp;_targetURL=https%3A%2F%2Fs100.copyright.com%2FAppDispatchServlet%3FpublisherName%3DELS%26contentID%3DS0304394004000370%26orderBeanReset%3Dtrue&amp;_acct=C000057889&amp;_version=1&amp;_userid=8842358&amp;md5=0aabc749708ac883169b4f9ed2e196a3\" rel=\"nofollow\" target=\"outwardLink\">Permissions &amp; Reprints<\/a><\/div>\n<\/li>\n<\/ul>\n<hr \/>\n<div>\n<h2 id=\"section_abstract\">Abstract<\/h2>\n<p>Histamine is known to excite a subset of C-fibers and cause itch sensation. Despite its well-defined excitatory action on sensory neurons, intracellular signaling mechanisms are not understood. Previously, we demonstrated that bradykinin excited sensory neurons by activating TRPV1 via the phospholipase A<sub>2<\/sub> (PLA<sub>2<\/sub>) and lipoxygenase (LO) pathway. We, thus, hypothesized that histamine excited sensory neurons via the PLA<sub>2<\/sub>\/LO\/TRPV1 pathway. Application of histamine elicited a rapid increase in intracellular Ca<sup>2+<\/sup> ([Ca<sup>2+<\/sup>]<sub>i<\/sub>) that desensitized slowly in cultured dorsal root ganglion neurons. Histamine-induced [Ca<sup>2+<\/sup>]<sub>i<\/sub> was dependent on extracellular Ca<sup>2+<\/sup> and inhibited by capsazepine and by SC0030, competitive antagonists of TRPV1. Quinacrine and nordihydroguaiaretic acid, a PLA<sub>2<\/sub> and an LO inhibitor, respectively, blocked the histamine-induced Ca<sup>2+<\/sup>influx in sensory neurons, while indomethacin (a cyclooxygenase inhibitor) did not. We thus conclude that histamine activates TRPV1 after stimulating the PLA<sub>2<\/sub>\/LO pathway, leading to the excitation of sensory neurons. These results further provide an idea for potential use of TRPV1 antagonists as anti-itch drugs.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>1-s2.0-S0304394004000370-main Histamine-induced Ca2+influx via the PLA2\/lipoxygenase\/TRPV1 pathway in rat sensory neurons \u2606 This paper is in honor of Manfred Zimmermann&#8217;s 70th birthday. Byung Moon Kim, Sang Hee Lee, Won Sik Shim, Uhtaek Oh, Sensory Research Center, National Creative Research Initiatives, College of Pharmacy, Seoul National University, San 56-1, Shinlim, Kwanak-Gu, Seoul 151-742, South Korea &nbsp; [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/92"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=92"}],"version-history":[{"count":7,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/92\/revisions"}],"predecessor-version":[{"id":203,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/92\/revisions\/203"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=92"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=92"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=92"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}