{"id":923,"date":"2015-01-16T10:31:50","date_gmt":"2015-01-16T01:31:50","guid":{"rendered":"http:\/\/misterx95.myds.me\/?p=923"},"modified":"2015-01-16T10:31:50","modified_gmt":"2015-01-16T01:31:50","slug":"journal-club-2015-1-16","status":"publish","type":"post","link":"https:\/\/misterx95.myds.me\/wordpress\/?p=923","title":{"rendered":"Journal Club 2015.1.16"},"content":{"rendered":"

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\r\n\t\r\n\t\t\"Cathepsin\r\n\t<\/a>\r\n<\/dt>\r\n
\r\n\tFilename<\/strong> : cathepsin-s-signals-via-par2-and-generates-a-novel-tethered-ligand-receptor-agonist.pdf<\/a> (789 KB)\r\n\t
\r\n\tCaption<\/strong> : \r\n<\/dd>\r\n<\/dl>

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Cathepsin<\/span> S<\/span> signals<\/span> via<\/span> PAR2<\/span> and generates<\/span> a novel<\/span> tethered<\/span> ligand<\/span> receptor<\/span> agonist<\/span>.<\/h1>\n
Elmariah SB<\/a>1<\/sup>, Reddy VB<\/a>1<\/sup>, Lerner EA<\/a>1<\/sup>.<\/div>\n
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Author information<\/span><\/a><\/h3>\n
<\/div>\n<\/div>\n
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Abstract<\/h3>\n
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Protease-activated receptor<\/span>-2 is widely expressed in mammalian epithelial, immune and neural tissues. Cleavage of PAR2<\/span> by serine proteases leads to self-activation of the receptor<\/span> by the tethered<\/span> ligand<\/span> SLIGRL. The contribution of other classes of proteases to PAR activation has not been studied in detail. Cathepsin<\/span> S<\/span> is a widely expressed cysteine protease that is upregulated in inflammatory conditions. It has been suggested thatcathepsin<\/span> S<\/span> activates PAR2<\/span>. However, cathepsin<\/span> S<\/span> activation of PAR2<\/span> has not been demonstrated directly nor has the potential mechanism of activation been identified. We show that cathepsin<\/span> S<\/span> cleaves near the N-terminus of PAR2<\/span> to expose a novel<\/span> tethered<\/span> ligand<\/span>, KVDGTS. The hexapeptide KVDGTS generates<\/span> downstream signaling events specific to PAR2<\/span> but is weaker than SLIGRL. Mutation of the cathepsin<\/span> S<\/span> cleavage site prevents receptor<\/span> activation by the protease while KVDGTS retains activity. In conclusion, the range of actions previously ascribed to cysteine cathepsins in general, and cathepsin<\/span> S<\/span> in particular, should be expanded to include molecular signaling. Such signaling may link together observations that had been attributed previously to PAR2<\/span> or cathepsin<\/span> S<\/span> individually. These interactions may contribute to inflammation.<\/p>\n<\/div>\n<\/div>\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Cathepsin S signals via PAR2 and generates a novel tethered ligand receptor agonist. Elmariah SB1, Reddy VB1, Lerner EA1. Author information Abstract Protease-activated receptor-2 is widely expressed in mammalian epithelial, immune and neural tissues. Cleavage of PAR2 by serine proteases leads to self-activation of the receptor by the tethered ligand SLIGRL. The contribution of other […]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/923"}],"collection":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=923"}],"version-history":[{"count":1,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/923\/revisions"}],"predecessor-version":[{"id":928,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/923\/revisions\/928"}],"wp:attachment":[{"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=923"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=923"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/misterx95.myds.me\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=923"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}