Publications

@article{Cha2024,

title = {Antipruritic effect of ursolic acid through MRGPRX2/MrgprB2-dependent inhibition of mast cell degranulation and reduced TSLP production},

author = {Jieun Cha and Juhee Ryu and Diwas Rawal and Wook-Joo Lee and Won-Sik Shim},

doi = {10.1016/j.ejphar.2024.176896},

issn = {0014-2999},

year  = {2024},

date = {2024-10-00},

urldate = {2024-10-00},

journal = {European Journal of Pharmacology},

volume = {981},

publisher = {Elsevier BV},

keywords = {Mast cell, MrgprB2, MRGPRX2, TSLP, Ursolic acid},

pubstate = {published},

tppubtype = {article}

}

@article{Adhikari2024,

title = {A phytosphingosine derivative mYG-II-6 inhibits histamine-mediated TRPV1 activation and MRGPRX2-dependent mast cell degranulation},

author = {Nisha Adhikari

Wook-Joo Lee

Soojun Park

Sanghee Kim 

Won-Sik Shim},

doi = {10.1016/j.intimp.2024.112113},

issn = {1567-5769},

year  = {2024},

date = {2024-05-00},

urldate = {2024-05-00},

journal = {International Immunopharmacology},

volume = {133},

publisher = {Elsevier BV},

keywords = {Histamine, Mast cell, MRGPRX2, TRPV1},

pubstate = {published},

tppubtype = {article}

}

@article{Adhikari2022,

title = {Caffeic acid phenethyl ester inhibits pseudo-allergic reactions via inhibition of MRGPRX2/MrgprB2-dependent mast cell degranulation},

author = {Nisha Adhikari and Won-Sik Shim},

doi = {10.1007/s12272-022-01405-2},

issn = {1976-3786},

year  = {2022},

date = {2022-09-00},

urldate = {2022-09-00},

journal = {Arch. Pharm. Res.},

volume = {45},

number = {9},

pages = {644--657},

publisher = {Springer Science and Business Media LLC},

abstract = {Mast cells play essential role in allergic reactions through the process called mast cell degranulation. Recent studies have found that a basic secretagogue compound 48/80 (C48/80) induces non-IgE-mediated mast cell degranulation via activation of human Mas-related G protein-coupled receptor X2 (MRGPRX2) and mouse MrgprB2. Although previous studies have revealed that caffeic acid (CA) and its derivatives possess anti-allergic effects via IgE-dependent manner, it is largely elusive whether these compounds have impact on MRGPRX2/MrgprB2 to exert inhibitory effects. Therefore, the present study investigated whether CA as well as its derivatives - rosmarinic acid (RA) and caffeic acid phenethyl ester (CAPE) - has the ability to inhibit the activity of MRGPRX2/MrgprB2 to evoke pseudo-allergic effects. As a result, it was found that CAPE inhibits C48/80-induced activation of MRGPRX2/MrgprB2, but neither CA nor RA showed discernible inhibition. Furthermore, the β-hexosaminidase release assay showed that CAPE inhibits mouse peritoneal mast cell degranulation in both IgE-dependent and MrgprB2-dependent manners. Additionally, mouse paw edema induced by C48/80 was dramatically suppressed by co-treatment of CAPE, suggesting that CAPE possesses a protective effect on C48/80-evoked pseudo-allergic reactions. The pretreatment of CAPE also significantly decreased scratching bouts of mice evoked by C48/80, demonstrating that CAPE also has an anti-pruritic effect. Therefore, these data implicate that CAPE can suppress pseudo-allergic reactions evoked by C48/80 via MrgprB2-dependent manner. Finally, molecular docking analysis showed that CAPE is predicted to bind to human MRGPRX2 in the region where C48/80 also binds, implying that CAPE can be a competitive inhibitor of MRGPRX2. In conclusion, it is found that CAPE has the ability to inhibit MRGPRX2/MrgprB2, leading to the prevention of mast cell degranulation and further to the alleviation of mast cell reactions. These results indicate that CAPE as a CA derivative could be developed as a new protective agent that exerts dual inhibition of mast cell degranulation mediated by IgE and MRGPRX2/MrgprB2.},

keywords = {Allergy, Calcium imaging, Mast cell, MrgprB2, MRGPRX2},

pubstate = {published},

tppubtype = {article}

}