List of Publications (2011-Current)
2020
Choi, Da-Som; Ji, Yeounjung; Jang, Yongwoo; Lee, Wook-Joo; Shim, Won-Sik
In: Biomolecules & Therapeutics, vol. 28, no. 6, pp. 569–575, 2020, ISSN: 2005-4483.
Abstract | Links | BibTeX | Tags: Calcium imaging, Chloroquine, H1R, Histamine, Itch, MrgprA3, TRPA1, TRPV1
@article{Choi2020,
title = {Crotamiton, an Anti-Scabies Agent, Suppresses Histamine- and Chloroquine-Induced Itch Pathways in Sensory Neurons and Alleviates Scratching in Mice},
author = {Da-Som Choi and Yeounjung Ji and Yongwoo Jang and Wook-Joo Lee and Won-Sik Shim},
doi = {10.4062/biomolther.2020.063},
issn = {2005-4483},
year = {2020},
date = {2020-11-01},
urldate = {2020-11-01},
journal = {Biomolecules & Therapeutics},
volume = {28},
number = {6},
pages = {569--575},
publisher = {The Korean Society of Applied Pharmacology},
abstract = {Crotamiton is an anti-scabies drug, but it was recently found that crotamiton also suppresses non-scabietic itching in mice. However, the underlying mechanism is largely unclear. Therefore, aim of the study is to investigate mechanisms of the anti-pruritic effect of crotamiton for non-scabietic itching. Histamine and chloroquine are used as non-scabietic pruritogens. The effect of crotamiton was identified using fluorometric intracellular calcium assays in HEK293T cells and primary cultured dorsal root ganglion (DRG) neurons. Further in vivo effect was evaluated by scratching behavior tests. Crotamiton strongly inhibited histamine-induced calcium influx in HEK293T cells, expressing both histamine receptor 1 (H1R) and transient receptor potential vanilloid 1 (TRPV1), as a model of histamine-induced itching. Similarly, it also blocked chloroquine-induced calcium influx in HEK293T cells, expressing both Mas-related G-protein-coupled receptor A3 (MRGPRA3) and transient receptor potential A1 (TRPA1), as a model of histamine-independent itching. Furthermore, crotamiton also suppressed both histamine- and chloroquine-induced calcium influx in primary cultures of mouse DRG. Additionally, crotamiton strongly suppressed histamine- and chloroquine-induced scratching in mice. Overall, it was found that crotamiton has an anti-pruritic effect against non-scabietic itching by histamine and chloroquine. Therefore, crotamiton may be used as a general anti-pruritic agent, irrespective of the presence of scabies.},
keywords = {Calcium imaging, Chloroquine, H1R, Histamine, Itch, MrgprA3, TRPA1, TRPV1},
pubstate = {published},
tppubtype = {article}
}
Crotamiton is an anti-scabies drug, but it was recently found that crotamiton also suppresses non-scabietic itching in mice. However, the underlying mechanism is largely unclear. Therefore, aim of the study is to investigate mechanisms of the anti-pruritic effect of crotamiton for non-scabietic itching. Histamine and chloroquine are used as non-scabietic pruritogens. The effect of crotamiton was identified using fluorometric intracellular calcium assays in HEK293T cells and primary cultured dorsal root ganglion (DRG) neurons. Further in vivo effect was evaluated by scratching behavior tests. Crotamiton strongly inhibited histamine-induced calcium influx in HEK293T cells, expressing both histamine receptor 1 (H1R) and transient receptor potential vanilloid 1 (TRPV1), as a model of histamine-induced itching. Similarly, it also blocked chloroquine-induced calcium influx in HEK293T cells, expressing both Mas-related G-protein-coupled receptor A3 (MRGPRA3) and transient receptor potential A1 (TRPA1), as a model of histamine-independent itching. Furthermore, crotamiton also suppressed both histamine- and chloroquine-induced calcium influx in primary cultures of mouse DRG. Additionally, crotamiton strongly suppressed histamine- and chloroquine-induced scratching in mice. Overall, it was found that crotamiton has an anti-pruritic effect against non-scabietic itching by histamine and chloroquine. Therefore, crotamiton may be used as a general anti-pruritic agent, irrespective of the presence of scabies.
2018
Lee, Wook-Joo; Kim, Young-Sik; Shim, Won-Sik
In: Journal of Ginseng Research, vol. 42, no. 4, pp. 470–475, 2018, ISSN: 1226-8453.
Abstract | Links | BibTeX | Tags: Calcium imaging, Chloroquine, Korean Red Ginseng, MrgprA3, Scratching behavior, TRPA1
@article{Lee2018,
title = {Korean Red Ginseng extract and ginsenoside Rg3 have anti-pruritic effects on chloroquine-induced itch by inhibition of MrgprA3/TRPA1-mediated pathway},
author = {Wook-Joo Lee and Young-Sik Kim and Won-Sik Shim},
doi = {10.1016/j.jgr.2017.05.004},
issn = {1226-8453},
year = {2018},
date = {2018-10-00},
urldate = {2018-10-00},
journal = {Journal of Ginseng Research},
volume = {42},
number = {4},
pages = {470--475},
publisher = {Elsevier BV},
abstract = {Background: It was previously found that Korean Red Ginseng water extract (KRGE) inhibits the histamine-induced itch signaling pathway in peripheral sensory neurons. Thus, in the present study, we investigated whether KRGE inhibited another distinctive itch pathway induced by chloroquine (CQ); a representative histamine-independent pathway mediated by MrgprA3 and TRPA1. Methods: Intracellular calcium changes were measured by the calcium imaging technique in theHEK293T cells transfected with both MrgprA3 and TRPA1 (“MrgprA3/TRPA1”), and in primary culture ofmouse dorsal root ganglia (DRGs). Mouse scratching behavior tests were performed to verify proposed antipruritic effects of KRGE and ginsenoside Rg3.Results: CQ-induced Ca2þ influx was strongly inhibited by KRGE (10 mg/mL) in MrgprA3/TRPA1, and notablyginsenoside Rg3 dose-dependently suppressed CQ-induced Ca2þ influx in MrgprA3/TRPA1. Moreover, bothKRGE (10 mg/mL) and Rg3 (100 mM) suppressed CQ-induced Ca2þ influx in primary culture of mouse DRGs,indicating that the inhibitory effect of KRGE was functional in peripheral sensory neurons. In vivo tests revealed that not only KRGE (100 mg) suppressed CQ-induced scratching in mice [bouts of scratching:274.0 51.47 (control) vs. 104.7 17.39 (KRGE)], but also Rg3 (1.5 mg) oral administration significantly reduced CQ-induced scratching as well [bouts of scratching: 216.8 33.73 (control) vs.115.7 20.94 (Rg3)]. Conclusion: The present study verified that KRGE and Rg3 have a strong antipruritic effect against CQ-induced itch. Thus, KRGE is as a promising antipruritic agent that blocks both histamine-dependent and -independent itch at peripheral sensory neuronal levels.},
keywords = {Calcium imaging, Chloroquine, Korean Red Ginseng, MrgprA3, Scratching behavior, TRPA1},
pubstate = {published},
tppubtype = {article}
}
Background: It was previously found that Korean Red Ginseng water extract (KRGE) inhibits the histamine-induced itch signaling pathway in peripheral sensory neurons. Thus, in the present study, we investigated whether KRGE inhibited another distinctive itch pathway induced by chloroquine (CQ); a representative histamine-independent pathway mediated by MrgprA3 and TRPA1. Methods: Intracellular calcium changes were measured by the calcium imaging technique in theHEK293T cells transfected with both MrgprA3 and TRPA1 (“MrgprA3/TRPA1”), and in primary culture ofmouse dorsal root ganglia (DRGs). Mouse scratching behavior tests were performed to verify proposed antipruritic effects of KRGE and ginsenoside Rg3.Results: CQ-induced Ca2þ influx was strongly inhibited by KRGE (10 mg/mL) in MrgprA3/TRPA1, and notablyginsenoside Rg3 dose-dependently suppressed CQ-induced Ca2þ influx in MrgprA3/TRPA1. Moreover, bothKRGE (10 mg/mL) and Rg3 (100 mM) suppressed CQ-induced Ca2þ influx in primary culture of mouse DRGs,indicating that the inhibitory effect of KRGE was functional in peripheral sensory neurons. In vivo tests revealed that not only KRGE (100 mg) suppressed CQ-induced scratching in mice [bouts of scratching:274.0 51.47 (control) vs. 104.7 17.39 (KRGE)], but also Rg3 (1.5 mg) oral administration significantly reduced CQ-induced scratching as well [bouts of scratching: 216.8 33.73 (control) vs.115.7 20.94 (Rg3)]. Conclusion: The present study verified that KRGE and Rg3 have a strong antipruritic effect against CQ-induced itch. Thus, KRGE is as a promising antipruritic agent that blocks both histamine-dependent and -independent itch at peripheral sensory neuronal levels.