List of Publications (2011-Current)
2018
Ji, Yeounjung; Jang, Yongwoo; Lee, Wook Joo; Yang, Young Duk; Shim, Won-Sik
Different perception levels of histamine-induced itch sensation in young adult mice Journal Article
In: Physiology & Behavior, vol. 188, pp. 188–193, 2018, ISSN: 0031-9384.
Abstract | Links | BibTeX | Tags: Dorsal root ganglia, H1R, Histamine, Itch, Scratching behavior, TLR4, TRPV1
@article{Ji2018,
title = {Different perception levels of histamine-induced itch sensation in young adult mice},
author = {Yeounjung Ji and Yongwoo Jang and Wook Joo Lee and Young Duk Yang and Won-Sik Shim},
doi = {10.1016/j.physbeh.2018.02.015},
issn = {0031-9384},
year = {2018},
date = {2018-05-00},
urldate = {2018-05-00},
journal = {Physiology & Behavior},
volume = {188},
pages = {188--193},
publisher = {Elsevier BV},
abstract = {Itch is an unpleasant sensation that evokes behavioral responses such as scratching the skin. Interestingly, it isconceived that the perception of itch sensation is influenced by age. Indeed, accumulating evidence supports theidea that even children or younger adults show distinctive itch sensation depending on age. This evidence implies the presence of a mechanism that regulates the perception of itch sensation in an age-dependent fashion. Therefore, the purpose of the present study was to investigate a putative mechanism for the age-dependent perception of itch sensation by comparing histamine-induced scratching behaviors in 45-day old (D45) and 75-day old male “young adult” mice. The results indicated that, following histamine administration, the D75 mice spent a longer time scratching than D45 mice. However, the intensity of the calcium influx induced by histamine in primary culture of dorsal root ganglia (DRG) neurons was not different between D45 and D75 mice. Moreover, no apparent difference was observed in mRNA levels of a characteristic His-related receptor and ion channel. Incontrast, the mRNA levels of Toll-Like Receptor 4 (TLR4) were increased approximately by two-fold in D75 DRG compared with D45 DRG. Additionally, D75-derived DRG neurons exhibited enhanced intracellular calcium increase by lipopolysaccharide (LPS, a TLR4 agonist) than those of D45 mice. Furthermore, intensities of calciuminflux induced by histamine were significantly potentiated when co-treated with LPS in D75 DRG neurons, butnot in those of D45 mice. Thus, it appears that D75 mice showed enhanced histamine-induced scratching behaviors not by increased expression levels of histamine-related genes, but probably due to augmented TLR4},
keywords = {Dorsal root ganglia, H1R, Histamine, Itch, Scratching behavior, TLR4, TRPV1},
pubstate = {published},
tppubtype = {article}
}
Itch is an unpleasant sensation that evokes behavioral responses such as scratching the skin. Interestingly, it isconceived that the perception of itch sensation is influenced by age. Indeed, accumulating evidence supports theidea that even children or younger adults show distinctive itch sensation depending on age. This evidence implies the presence of a mechanism that regulates the perception of itch sensation in an age-dependent fashion. Therefore, the purpose of the present study was to investigate a putative mechanism for the age-dependent perception of itch sensation by comparing histamine-induced scratching behaviors in 45-day old (D45) and 75-day old male “young adult” mice. The results indicated that, following histamine administration, the D75 mice spent a longer time scratching than D45 mice. However, the intensity of the calcium influx induced by histamine in primary culture of dorsal root ganglia (DRG) neurons was not different between D45 and D75 mice. Moreover, no apparent difference was observed in mRNA levels of a characteristic His-related receptor and ion channel. Incontrast, the mRNA levels of Toll-Like Receptor 4 (TLR4) were increased approximately by two-fold in D75 DRG compared with D45 DRG. Additionally, D75-derived DRG neurons exhibited enhanced intracellular calcium increase by lipopolysaccharide (LPS, a TLR4 agonist) than those of D45 mice. Furthermore, intensities of calciuminflux induced by histamine were significantly potentiated when co-treated with LPS in D75 DRG neurons, butnot in those of D45 mice. Thus, it appears that D75 mice showed enhanced histamine-induced scratching behaviors not by increased expression levels of histamine-related genes, but probably due to augmented TLR4