Scratch-AID, a deep learning basedsystem for automatic detection of mousescratching behavior with high accuracy

Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States; 2
Graduate Group in Biostatistics, University of California Davis, Davis, United States; 3 Howard Hughes Medical Institute,
Program in Cellular and Molecular Medicine, Boston Children’s Hospital, Harvard Medical School, Boston, United States; 4 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, United States; 5 Department of Molecular and Cellular Biology, University of California Davis, Davis,
United States; 6 Department of Mathematics, University of California Davis, Davis, United States

Abstract

Mice are the most commonly used model animals for itch research and for development of anti-itch drugs. Most laboratories manually quantify mouse scratching behavior to assess itch intensity. This process is labor-intensive and limits large-scale genetic or drug screenings. In this study, we developed a new system, Scratch-AID (Automatic Itch Detection), which could automatically identify and quantify mouse scratching behavior with high accuracy. Our system included a custom-designed videotaping box to ensure high-quality and replicable mouse behavior recording and a convolutional recurrent neural network trained with frame-labeled mouse scratching behavior videos, induced by nape injection of chloroquine. The best trained network achieved 97.6% recall and 96.9% precision on previously unseen test videos. Remarkably, Scratch-AID could reliably identify scratching behavior in other major mouse itch models, including the acute cheek model, the histaminergic model, and a chronic itch model. Moreover, our system detected significant differences in scratching behavior between control and mice treated with an anti-itch drug. Taken together, we have established a novel deep learning-based system that could replace manual quantification for mouse scratching behavior in different itch models and for drug screening.

Presenter: Gi Baek Lee