5-HT3 receptors antagonists reduce serotonin-induced scratching in mice
Running title: 5-HT3 receptors mediate serotonin-induced scratching
Sattar Ostadhadi a,b, Nastaran Kordjazy a,b, Arya Haj-Mirzaian a,b, Parvin Mansouri c, Ahmad Reza Dehpour a,b*
a Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
b Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
c Skin and Stem cell Research Center, Tehran University of Medical Sciences, Tehran, Iran
* Corresponding author:
Prof. Ahmad Reza Dehpour, PharmD, PhD, Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, P.O. Box 13145-784, Tehran, Iran. Tel: +98 21 88973652, fax: + 98 21 66402569, e-mail: dehpoura@sina.tums.ac.ir
ABSTRACT
Serotonin (5-hydroxytryptamine, 5-HT) acts as a pruritogen in humans and animals, but the mechanisms of action through that serotonin induces itch-response have not been extensively discovered. In our study, we attempted to investigate the role of 5-HT3 receptors in scratching behavior due to intradermal serotonin injection. Intradermal injection of serotonin (14.1–235 nmol/site) into the nape of the neck of mice was performed to elicit itch. Scratching behavior was evaluated by measuring the number of bouts during 60 minutes after injection. We evaluated the effect of intraperitoneal pretreatment with ondansetron and tropisetron (0.1, 0.3, and 1 mg/kg) on itch induced by serotonin. Also, intradermal ondansetron and tropisetron at doses 50, 100, and 200 nmol/site were concurrently administrated with serotonin. Serotonin produced a significant enhancement in scratching at dose 141 nmol/site. Concurrent administration of ondansetron (50, 100, and 200 nmol/site) and tropisetron (100 and 200 nmol/site) with serotonin reduced scratching activity compared to the animals that only received serotonin. Also, pretreatment with intraperitoneal ondansetron and tropisetron (0.3 and 1 mg/kg) 30 min before serotonin attenuated the itch response. We showed that the scratching induced by intradermal serotonin is mediated by 5-HT3 receptors subtype. It can be concluded that 5-HT3 may play a role in mediating serotonin-associated itch responses, and we introduce 5-HT3 receptors as possible targets for antipruritic agents.
Key words: Scratching; Serotonin (5-HT); 5-HT3 antagonists; Mice
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